Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
Premium Transformation Trigraph Escherichia coli
Ravindra Nandigam‚ Ph. D.‚ South Texas College Name: Course Number: Semester: Phys 1417 Physical Science II‚ Homework #5 Instructions: 1. Type your name‚ Course number (=PHYS 1417.V01)‚ and Semester (=Summer I‚ 2013). 2. Type your answers. Include questions. 3. Make sure that all parts of each question are answered. 4. Turn in. Chapter 25 Rocks and Minerals 1. List the 8 elements which make up about 98% of Earth’s entire mass. 2. The most abundant element in the Earth’s interior is __________________
Premium Plate tectonics Sedimentary rock
Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
Premium Enzyme PH Buffer solution
Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
Premium Protein Amino acid
Page 1/3 09/2010 SE 110.22 Forces in an Overdeterminate Truss The picture shows SE 110.22 in a frame similar to SE 112. * Comparison of forces in statically determinate and overdeterminate trusses1 * Bars with strain gauge full bridges to measure bar force1 * Computerised evaluation of experiments Technical Description Overdeterminate trusses are employed where overdimensioning is purposely required because safety must be maintained in the event of failure of an element‚ such as in aircraft
Premium Beam Force Truss
Purpose To check whether mass is gained or lost during a Chemical reaction. Hypothesis Equipments and Materials • Eye protection • Test tube • 250 ml Erlenmeyer flask and stopper • Weight Balance • Antacid tablet • Dilute solutions of o Sodium Hydroxide‚ NaOH(aq) o Iron(III) Nitrate‚ Fe(NO3)3 • Funnel • Cylinder Procedures 1. Put eye protection on for safety purposes. Part A: Reaction between Iron(III) Nitrate and Sodium Hydroxide. 2. Take two cylinders and fill one (full)
Free Sodium hydroxide Chemistry Sodium
Qualitative Analysis Shaopeng Zhang January 26‚ 2015 I submit this laboratory report as an original document. I assert that all ideas and discussion of data contained herein is my own work unless otherwise referenced. Tessa Landauer Abstract The goal of the experiment was to isolate and purify the unknown D liquid and solid by using its acidic and basic characteristics in a chemically active extraction then to identify the unknowns by analyzing the physical properties on IR and NMR spectrums. The neutral
Premium Solvent Temperature Chemistry
For this lab‚ the expected outcomes are that when the heart rate and blood pressure are measured‚ both will increase when the heart has to pump against gravity‚ after exercise the heart rate will increase then return to normal‚ and as the subject inhales‚ it will be lower than when they exhale. Methods For this lab report‚ the laboratory manual (2009) was used for all labs. While completing lab five‚ electrodes were hooked up to the subject. After calibration for this lab‚
Premium Blood Heart Respiratory system
The Determination of the Concentration of Hard Water Ions by the Titration of EDTA By Hannah Denby Lab partners: Heidi Kiziah‚ Leonie Hamel University of Central Florida‚ CHM 2046L December 3rd‚ 2015 Abstract. The “hardness” of water is a common dilemma attributed by high concentrations of metals such as calcium and magnesium. This is a result of the properties of water; because it is a good solvent‚ impurities are easily dissolved. As water moves through soil and rock‚ it dissolves minute amounts
Premium Water Chemistry Titration
MATERIALS : METALS AND NON-METALS Y Iron Coal ou are familiar with a number of materials like iron‚ aluminium‚ copper‚ etc. Some materials have been given in Table 4.1. Table 4.1 : Appearance and Hardness of materials Object /Material Appearance Hardness (Shiny/Dull) (Very hard/ Not very hard) similar change if we try to beat a wood log ? Let us find out. Activity 4.1 Take a small iron nail‚ a coal piece‚ a piece of thick aluminium wire and a pencil lead. Beat the iron nail with
Premium Oxygen Sulfuric acid Copper