MIC 230 Unknown Identification Report The objective of this experiment was to identify an organism from a mixture of two unknown bacterial species. In order to accomplish this‚ I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully
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MAHOGANY MAGIC POLISH an investigatory project submitted as an entry to the DIVISION SCIENCE FAIR 2012-2013 (LIFE SCIENCE) Submitted By: PAULINE G. LUDIVICO SHERYL ANNE H. PRESTOZA JOBERT JAN B. PICHUELA ANJULI E. ESTARES Coach Pag-asa National High School City Schools Division of Dasmariñas Region IV-CALABARZON Abstract Mahogany Magic Polish is made from mahogany seed hull charcoal‚ beeswax‚ virgin coconut oil‚ olive oil and vinegar. This polish produces a different
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experiment is to become familiar with the specific microbiological technique known as the aseptic technique‚ which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining to his doctor about pain in his lower abdominal and back. With a family history of prostate cancer‚ he worried that his sudden need to urinate
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In our daily diets‚ carbohydrates‚ fats‚ proteins and water are the main components in our food. However‚ if the food is left untreated‚ oxidative deterioration of the flavor and odor of fats and fatty constituents in it will be resulted. Hence‚ antioxidant are used widely in the production of food which fats and oils are used as raw materials and in the marketing of foods containing fats under modern conditions. The major factor in quality degradation of fats and fatty portions of foods is oxidation
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finding out I was negative for Indole‚ looking at my key my next test was Phenol Red Arabinose which also put me at a four to two ratio for finding out my unknown. After performing this test I found out this test is also negative. I recorded this test as negative because there was no color change in the tube meaning no reaction/no fermentation. Phenol Red Broth is a differential medium which also has a carbohydrate added. Phenol red and peptone are included in the medium as ph indicators; an indicator
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2Br2 ==> Br2RC-CRBr2R = H‚ alkyl or arylSaturated alkanes give no fast reaction with bromine. | Hydroxy group R-OH in alcohols and phenols (in ’dry’ conditions*) The first 3 tests (i) - (iii) given on the right are quite general for most alcohols. | (i) Mix it with a few drops of ethanoyl chloride‚ test fumes with litmus and silver nitrate (* note ethanoyl chloride reacts with water‚ phenols and amines too!).(ii) Mix it with a little phosphorus(V) chloride and test as above.(iii) Warm with a little ethanoic acid
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Tertiary( none Phenols Reaction with bromine/water (Confirms if phenol compound) Reagent: bromine in H2O Observation: Disappearance of orange-brown bromine color‚ then formation of white precipitate Procedure: 3 drops phenol soln on test tube+ Br in H2O drop by drop while shaking‚ observe formation of ppt Oxidation by KMnO4 (Confirms if phenol compound) Reagent: aqueous neutral KMnO4 Observation: purple color( brown color (formation of quinone) Procedure: 3 drops phenol soln on test tube+
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negative control‚ noinhibition zone was observed in control. Our investigation showed that the antibacterial activity shown by the aqueous leaf extracts of the Annona squamosa Linn. plants is possibly due to the presence of phytochemicals i.e tannins‚ phenols and
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solvent‚ use bromothymol blue as the indicator‚ since it gives a sharper endpoint than phenolphthalein. Te equivalent weight can be calculated from the following equation: B. ALCOHOLS 1. Ceric Nitrate – A qualitative test for alcohols and phenols. a. For Water-Soluble Compounds Add 0.5 mL of the ceric nitrate reagent to 2-3 mL of distilled water in a test tube‚ and mix thoroughly. Add 1 to 2 drops of the compound to be tested‚ shake‚ and note the color. If the
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UNIVERSITY OF THE GAMBIA LECTURE NOTES COURSE: PRINCIPLES OF CHEMISTRY II (ORGANIC CHEMISTRY) CODE: CHM 161 2ND SEMESTER SESSION: 2012/2013 LECTURER: ANTHONY F. ADJIVON UNIT 1 INTRODUCTION Organic chemistry started as the chemistry of life‚ when that was thought to be different from the chemistry in the laboratory. Then it became the chemistry of carbon compounds‚ especially those found in coal. Now it is both. It is the chemistry of the compounds of carbon along with other elements
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