Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Chromatography Lab Questions Pre-Lab Questions 1. A pencil is used rather than a pen to mark the “starting line” in step two because a pen or marker while be used to place a small dot of ink in the center of the X that is made with a pencil. 2. Capillary action is filling of pores in a solid with a liquid. Capillary action is caused by spotting or absorption and is allowed to seep or flow through. 3. The type of pen that will probably give better separation in this paper chromatography
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Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*‚ Phoebe Abalos‚ Kevin Antiga‚ Carmelus Aseneta 3-Biology 2 College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted
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Jessica Zebracki Organic Chem II Lab 2/15/15 Friedel-Crafts Acylation of Ferrocene and Column Chromatography Introduction: The intention of this lab is to analyze the formation of acetylferrocene using column chromatography. The Friedel-Crafts acylation reaction created acetylferrocene and diacetylferrocene‚ using phosphoric acid as a catalyst for the reaction between acetic anhydride and ferrocene (once applying heat). During column chromatography‚ a solution is passed through a filtration system
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In 1929 former Disney animators Hugh Harmanﾧ and Rudolf Isingﾧ made a cartoon entitled Bosko‚ the Talk-Ink Kidﾧ‚ and tried to sell it to a distributor in 1930. Warner Bros.ﾧ who had previously tried an unsuccessful attempt to set up a cartoon studio in New York in order to compete with Disney‚ agreed to distribute the series. Under producer Leon Schlesingerﾧ’s guide Harman and Ising created Looney Tunesﾧ (the title being variation on Disney’s Silly Symphonies) starring their character Boskoﾧ. A second
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The Potential of Tannin Found in Avocado Seeds (Persea Americana) As Alternative Source of Ink A Science Investigatory Project in Partial Fulfillment for the Requirement in Science IV Presented to: Ms. Hazel M. De Ramos Science IV Teacher Presented by: Española‚ Daveriel P. Misolas‚ Kim Zeus N. Sta. Ana‚ Rhoel Marvin O. Discaya‚ DoumaJairah V. Orgado‚ Nadine Gladys B. Polytechnic University of the Philippines Laboratory High School February 2014 Table of Contents Chapter I: The Problem
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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ALCOHOL WITH CHARCOAL AS SUBSTITUTE TO PENTEL PEN INK An Investigatory Project Presented to The Faculty of the High School Department Surigao Education Center Km. 2‚ Surigao City ____________________________________ In Partial Fulfillment of the Requirements For Science III ____________________________________ by Alutaya‚ Klaus Steven Kinkel G. Guy-ab‚ Jayford Jumandos‚ Julie Ann Cabuyaon‚ Laica Chua‚ Joshua Jordan . Roma‚ Jaypaul March 2013 ii ACKNOWLEDGEMENT Whatever
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