Experiment 5 Analysis of Analgesic Tablet by High Performance Liquid Chromatography Abstract An unknown sample‚ 529‚ was tested using high performance liquid chromatography to detect the concentrations of acetaminophen‚ aspirin‚ and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen‚ 11.5±0.185mg/100mL of aspirin‚ and 4.89±0.185mg/100mL of caffeine. Based on accepted values‚ the maximum daily amounts of each compound are 4000mg of acetaminophen
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Stroop was interested in the effects of interference and he tested it using colors‚ blocks and symbols to do this. Stroop used names of colors printed in incongruent ink‚ the same color ink‚ and in black ink to see if there was an effect that could show interference. He wanted to know how interference could be effected by stimuli like color and symbols. By looking at other research that was done before he dove into his studies on interference‚ he was able to gain insight on the subject. One of them
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Krotec; Honors Bio Period 4 Photosynthesis Lab Report I. Introduction The photosynthesis lab is designed to quantify photosynthesis‚ enabling a number of variables to be tested for their effects on photosynthetic rate. The production of oxygen‚ is used as an indirect measure of photosynthetic activity.The lab allows the examination of the effects of different wavelengths of light on photosynthetic activity. The other aspect of the lab includes Paper chromatography‚ which is a useful technique for separating and identifying pigments and
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will broaden range &may increase melting point Lab Questions 1. Define the following A. Melting point B. Intermolecular forces C. Hydrogen bonding 2. Arrange the following IMF according to there strength Dipole-Dipole interaction forces‚ Hydrogen bonding‚ Van der Waals forces 3. How does the atmospheric pressure affect the melting point? 4. What is the purpose of determining the melting point in this lab? 5. Why is this method not used for finding the melting
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PAPER AND THIN-LAYER CHROMATOGRAPHY Lab 47 Michael Stravino‚ CHM 2211L‚ June 3‚ 2012 Abstract Thin-Layer Chromatography and Paper Chromatography can be used to identify identical compounds‚ unknown compounds‚ the number of components in a mixture‚ as well as other important uses not explored in this laboratory. This experiment explores these techniques to identify an unknown amino acid by TLC and analyze food colors using paper chromatography. By using 2.0 mL of dimethoxyethane and 18 mL of
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blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
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Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as
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Lab: investigating hooked law with springs Purpose: to find spring constants of different springs using the slope of a graph of change in heights vs. the weight force. Also‚ to be able to understand how spring constants change when you add springs in a series or paralle Pre lab predictions: We predicted that the graph of gravitational force (mg) as a function of stretch (delta x) would look like Data: Spring #1: y = 8.2941x + 0.0685 This table represents the different distances that
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Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
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The goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used
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