PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
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indicated by a very pale pink color. To calculate the molarity of NaOH‚ the following equation was used MNaOH x VNaOH = MKHP x VKHP therefore the molarity was .125 M. INTRODUCTION This lab experiment covers the preparation of standard solution and the acid/base titration. The first part of the lab is to prepare a standard solution of Potassium hydrogen per. A standard solution is a solution of known concentration‚ in which it is prepared using exacting techniques to make sure that the molarity
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Lab #3 Separating Metal Cations by Paper Chromatography Introduction/Purpose: In chemistry‚ it is important to effectively analyze the components in a given mixture. In order to do so‚ chemists will analyze a mixture either qualitatively or quantitatively using different methods and equipment. Qualitative analyses are subjective‚ and only gather “non-numerical” data such as the color‚ appearance‚ and smell of the components. This data
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Analysis of Essential Oils using Gas Chromatography Lyndon Justin T. Guzman Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Date Performed: February 2; February 4‚ 2011 Date Submitted: February 18‚ 2011 Abstract The purpose of this experiment is to isolate the essential oil from eucalyptus leaves as a pure compound; moreover‚ the components of the essential oil‚ camphor and limonene‚ will be then separated using gas chromatography technique‚ identify the components by
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Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
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...................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays .............................................................
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A. What is the name of the pigment that captures light? Chlorophyll B. Why does the pigment appear green? It absorbs other colors of light such as red and blue‚ so in a way the green light is reflected out since the pigment does not absorb it. 2. List two variables besides the wavelength (color) of light which might affect the rate of food production in plants. Light intensity and carbon dioxide 3. Why is chlorophyll important for all biological life? It is necessary for photosynthesis which
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seen at the top of the solution. Lead Acetate Cloudy solution with small specks of white precipitate. Ice cold ethanol Cloudy at the bottom and about 0.5 cm of the top of the solution was clear. Table 4: The results of Gel Filtration Chromatography Eluate Component Volume collected in mL First Blue dextrin 3.00 Second Brown cytochrome 7.00 Third Clear phosphate buffer 5.00 Table 5: The result of Electrophoresis of Amino Acids Distance
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Nervous System lab was designed to measure reaction times by using a variety of tests that affect the heart rate. These tests were the startle‚ stroop‚ Valsalva maneuver‚ diving reflex‚ and a test of our choice. The test our group chose to measure the heart rate in response to doing a trust fall to anticipate the stress right before the fall. To begin this lab we chose a participant‚ and then hooked their finger up to the pulse transducer sensor. Using the chart 5 program on the lab computer the participant’s
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