of antigen above antiserum. 5. Include appropriate positive and negative specimen controls. Reagents used: * Acetic acid * Saline containing 0.5% phenol * Anthrax antiserum: Antiserum is prepared in rabbits by the subcutaneous Inoculation of Sterne anthrax vaccine on days 1 and 14. On days 28 and 35‚ the rabbits receive 0.5 mL of a mixture of several strains of virulent B. anthracis not exceeding 105 colony-forming units (CFU)/ml suspended in saline. Alternatively‚ the live virulent
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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Autumn White Biology 258-05 November 26‚ 2012 Unknown #19 Enterbacteriaceae Enterobacter aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the
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MUSHROOM CULTIVATION AT TAR COLLEGE CASE STUDY JOHORE BRANCH CAMPUS This case was prepared by Low Kock Hee‚ Senior Lecturer School of Business Studies TAR COLLEGE This case study was written for the purpose of class discussion. The case was neither designed nor meant to illustrate the correct or incorrect management of the problems or issues contained in the case. ACKNOWLEDGEMENTS The author of this case study thanks Mr. Liaw Fenn Yenn‚ Head‚ TAR College‚ Johore Branch and the campus staff
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separate TSA plates by swabbing the agar in a zigzag fashion‚ rotating the petri dish to spread the bacteria in various directions. This culturing technique helped us to see the full growth of the biofilm that would be growing after inoculation. After the fifth day of inoculation‚ we were able to visibly see the various bacterial colonies of our biofilms. We proceeded to gram-stain a colony from each biofilm‚ in search of colonies of gram-positive bacteria. There are very specific steps that must be taken
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The Childhood Vaccination Debate Student’s Name Eng/147 Today’s Date Teacher’s Name The Childhood Vaccination Debate Imagine a disease that begins much like the flu but ends with painful fluid filled pustules covering large portions of one’s body that can rob one’s ability to see‚ eat‚ and breathe. Furthermore‚ according to Berkeley University’s news writer‚ David Koplow (2003) this disease “has caused more deaths than all the world wars combined” (para. 3). Now‚ imagine if one quick vaccination
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Unknown Report Emily Juhl Section – 3 April 15‚ 2013 Unknown #8 Family: Enterobacteriaceae Genus: Serratia Species: Marcescens Abstract Microorganisms are constantly finding ways to resists to antibiotics. For this reason‚ it is important to test and observe unknown organisms in the lab to continually improve the health and well being of society. The objective of this report was to first isolate a single colony of an unknown culture on a LB agar plate. From
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The agar contains Triphenyl tetrazoliumchloric (TTC) which gives off a color indicator for motility. If the organism moves from the stab indention‚ then the inoculation shows it was motile and grew red in the agar. However‚ if the organism did not move from the stab indention‚ then it will appear just a thin precipitate of red‚ which indicates a non-motile growth. Compare the motility with the positive control
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BHS002-1 Microbiology Practical Manipulation of bacteria – Part 1 Week 45 Since the early days of microbiology in the 19th century‚ culture on agar plates has been a central technique for the study of bacteria. This practical is designed to introduce students to the basic techniques required to manipulate bacteria. Students will gain experience with the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread
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promotional mix are: personal selling‚ advertising‚ publicity and the web True False 5 points Question 2 1. A major advantage to two sided messages is the inoculation effect it has for the receivers to competitive messages True False 5 points Question 3 1. A major advantage to two sided messages is the inoculation effect it has for the receivers to competitive messages True False 5 points Question 4 1. When considering the channel in the communication process the major
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