Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Determination is a characteristic very few people have. But‚ in this case the main character has this uncommon attribute. In the book Through my Eyes (Tebow)‚ Tim‚ a determined football player‚ shows a plethora of events of him never giving up. Tim Tebow never gave up on anything‚ including his education‚ football career‚ and farming. School work was especially challenging for Tim considering that he was diagnosed with dyslexia as a child. Isn’t it hard to imagine getting a good education with
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Determination of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96‚ and the pKa’s found experimentally were 2.0‚ 4.0‚ and 9.85. The accepted pKa values were found to be 2.10‚ 4.07‚ and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid‚
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Emilia Monroe August 27‚ 2014 Experiment #01 The Determination of the Percent of Water in a Compound CHEM 1315-022 For experiment one‚ The Determination of the Percent of Water in a Compound‚ the sole purpose of conducting this experiment was to determine the percent of water found in compounds such as Magnesium Sulfate‚ Copper Sulfate‚ and so on. Along with determining the percentages of the hygroscopic compounds the experiment allowed for the exploration of separation of hydrogen bonds
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2.4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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1.0 Title Determination of Protein Content Using Kjedahl and Titration 2.0 Introduction Proteins are polymers. They are the source of dietary amino acids and are used for growth and maintenance of living systems. They are costlier sources of energy compared to carbohydrates and fats and hence the human body utilizes proteins mainly for biosynthesis rather than as an energy source‚ though the energy yield is 5 kcal/g of protein. Twenty different types of amino acids occur naturally in proteins
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was self-determination‚ the belief that all people have the right to choose their political destiny free from the interference of any external parties. Naturally‚ the announcement of self-determination was met with a wave of optimism amongst colonised nations like Vietnam‚ Poland‚ India‚ and Egypt. Yet this optimism was quickly stamped out by the Wilson administration’s inconsistent application of self-determination. As a result‚ this essay asserts that in Wilson’s mind‚ self-determination only applied
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Determination of Calcium ions in milk using titration. Background Calcium is a mineral which is essential to the human body. In fact 1.5% of the human body is made up of calcium‚ and not just the obvious uses such as bone and teeth formation but it is also a vital factor in many enzyme reactions‚ for example blood clotting. It also partakes in the regulation of the hearts rhythm. Lack of the crucial mineral can result in the build up of cadmium and lead‚ both of which are toxic. along
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Experiment 5- Standardization of NaOH and determination of Molarity of an unknown Acid Objectives 1. Preparation and standardization of a 0.1M NaOH solution 2. To learn the technique of titration 3. Determination of the concentration of an unknown diprotic acid. Introduction Titration can be traced to the origins of volumetric analysis‚ which began in the late eighteenth century. Study of analytical chemistry began in France and the first burette was made by Francois Antoine Henri
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Thermochemistry: An Ice Calorimeter Determination of Reaction Enthalpy Shannon Wedepohl 9/17/13 Abstract: During this experiment‚ an ice calorimeter was used to measure the change in volume in milliliters of magnesium metal and 1.00 M of sulfuric acid. We found the experimental molar enthalpy of the reaction to be ∆H = -370 ± 1 kJ/mol at 0˚C. This compares with an expected value of -466.9 kJ/mol at 25 ˚C‚ a 20.75% difference. Introduction: Many chemical reactions truly only
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