I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a solid membrane (PVDF or Nitrocellulose)
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PROTIEN Question (1): Define and distinguish primary structure‚ secondary and tertiary structures of proteins. Protein Structures: Primary structure Primary structure of protein is its unique sequence of amino acids forming its polypeptide chain. The primary structure of a protein is starting from the amino-terminal (N) end to the carboxyl-terminal (C). Secondary structure Most proteins have segments of their polypeptide chain repeatedly coiled of folded in patterns. These coiled & folded
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DNA safe and intact. The nuclear envelope regulates the flow of substances into and out of the nucleus. The NUCLEOLUS is an important structure found within the nucleus. The RNA‚ which will be made into ribosomes is synthesised in the nucleolus. The ribosomes move out of the nucleus to help with protein synthesis. The ROUGH EDOPLASMIC RETICULUM looks rather “pebbeled” through a microscope because of the ribosome’s that are present on the surface. The ribosomes have under gone protein synthesis and
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pierced with tip of knife‚ turning over once. (If you have a grill with widely spaced grates‚ you may want to place burgers on a perforated grill topper to keep them intact.) Place burgers on warmed buns. Serve with cucumber slices‚ lettuce leaves‚ and green onions if you like. Each serving: About 275 calories‚ 30 g protein‚ 24 g carbohydrate‚ 5 g total fat (1 g saturated)‚ 2 g fiber‚ 72 mg cholesterol‚ 310 mg sodium. Teriyaki Burgers: Prepare and cook Basic Burgers as above‚ but in
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of phospholipids and proteins which contribute to its selectively permeable nature. The function and permeability of the cell membrane depends on its intact structure. When destroyed‚ the permeability of the cell membrane is disrupted causing cellular contents to leak out. The cell membrane can be destroyed by physical damage‚ chemicals and high temperature. When cells are cut‚ the cell membranes are mechanically ruptured. High temperature disrupts the structure of proteins and certain chemicals
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The flour produced from the cassava plant‚ which on account of its low content of noncarbohydrate constituents might well be called a starch‚ is known in world trade as tapioca flour. It is used directly‚ made into a group of baked or gelatinized products or manufactured into glucose‚ dextrins and other products. Starchy foods have always been one of the staples of the human diet. They are mostly consumed in starch-bearing plants or in foods to which commercial starch or its derivatives have been
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destruction. (Cancer is discussed on pp. 145-146) 3.2 Selective permeability is a characteristics of healthy‚ intact cells. When a cell (or its plasma membrane) is severely damaged‚ the membrane becomes permeable to virtually everything‚ and substances flow into and out of the cell freely. This phenomenon is evident when someone has been severely burned. Precious fluids‚ proteins‚ and ions "weep" from the damaged cells. 3.3 Hypertonic solutions are sometimes infused intravenously into the
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Cell Membrane – The cell membrane protects the inside of the cell from outside containments‚ keeps the cell in contact and maintains its shape. This relates to mall security guards. They’re job is to protect the mall‚ make sure everything is intact and everybody is safe. Without the cell membrane‚ containments could enter‚ the security guards do the same. Without them‚ bad people or things could happen in the mall. Cell Wall – The cell wall provides structural support and protection to the cells
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oxide precipitate. turbidimetric method protein in urine Quantitative Tests for 24-Hour Specimens.Trichloroacetic acid (TCA) test. The addition of TCA to a urine specimen precipitates the protein in a fine suspension that is quantified spectrophotometrically at 420 nm (nanometers) by comparison with a similarly treated standard. Heat and acetic acid method Heat test: Based on the principle of heat coagulation and precipitation of proteins. If any turbidity appears‚ add 2 drops of
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trachomatis encode the inclusion membrane proteins; Npt1 and Npt2 (Nucleoside triphosphate transport protein). The Npt1 facilitates the import of host cell ATP coupled to the export of ADP while Npt2 imports ATP and other nucleotides (CTP‚ GTP and UTP) in a proton dependent fashion [8‚ 9]. Npt1 has been found in both EB and
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