Anchorage Dependent Animal Cell Culture Animal Cell Culture: Its origin and importance Prior to discussing about the anchorage dependence of animal cell cultures‚ let us first briefly discuss animal cell culture in general and its importance. Animal cell culture has a history of over 100 years when the first attempts to grow cells were made by Ross Harrison who was able to show the development of nerve fibres from frog embryo tissue cultured in a blood clot. Physiologist Alexis Carrel was able
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Contraction on Acceleration Signal in Human Skeletal Muscle. Department of Sport Sciences‚ Japan Institute of Sports Science‚3-15-1 * Colombinim.B‚ Nocella.M‚ Benelli.G‚ Giovanni.C‚and Bagni.M.A.(2008). Effect of temperature on cross-bridge properties in intact frog muscle fibers. Departimento di Scienze Fisiologiche and Istituto Interuniversitario di Miologia. Unicersita degli Studi di Firenzem Firenze‚ Italy.
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MALE REPRODUCTION MALE REPRODUCTIVE PHYSIOLOGY Testis - 2 compartments: interstitial tissue containing the Leydig cells that synthesize and secrete testosterone and the seminiferous tubules that produce spermatozoa and contain Sertoli cells Leydig cells and testosterone synthesis - Leydig cells mainly produce testosterone - T is a prohormone for synthesis of estradiol (vs. aromatase) and DHT (via. 5-alpha-reductase) - many effects of T are mediated via estradiol (esp in
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2011 Marfan syndrome is a variable‚ autosomal-dominant disorder in the connective tissue with distinct physical characteristics. The principal features affect the cardiovascular system‚ eyes and skeleton. This condition affects the connective protein that forms the structural support for tissues outside the cell. This disease is a result of a faulty makeup of chromosomes. Since MFS (Marfan Syndrome) is an autosomal-dominant disorder‚ only one copy of a faulty gene is necessary to cause manifestation
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Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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the compound to be gas at room temperature. The network of hydrogen atoms links all the water molecules together which makes it much more difficult to escape. This property gives the water cohesiveness. A drop of water on a polished surface stays intact and hardly wets the surface however a drop of alcohol on the same surface just spreads across it. Relating to the theme the importance of this aspect is to
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development and fatigue. There can also be a rapid increasing weakness in legs‚ pelvis‚ arms‚ and neck. Learning disabilities occur in about one-third of those diagnosed with DMD. It is caused by a mutation in the DNA that codes for dystrophin. This is a protein that helps
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Ch.2 Essential chemistry for Biology 1. Water is precious to life because living organisms use water as the medium (solvent) for chemical reactions necessary to sustain life. Water also helps maintain temperatures. Droughts are disastrous because they can wipe out crops‚ which in turn cause famine. 2. MATTER: Composed of chemical elements‚ occupies space and has mass (ex: solid‚ liquid‚ and gas states.) CHEMICAL ELEMENTS: substance that cannot be broken down into other substances by chemical reactions
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Introduction AG is 50yo female with a history of Sclerosing Mesenteritits‚ diabetes‚ hypothyroidism‚ smoking and alcohol abuse. She was admitted on 1-27-12 for severe abdominal pain along with nausea and vomiting. CT scan of the abdomen revealed a massive gastric dilation and thickening of the duodenal wall leading to occlusion of the duodenal lumen. I met AG on 2-23-12 when she was three weeks post-operative (post-op)‚ gastrojejunostomy‚ resection of the pancreatic head mass biopsy‚ and
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over the past three months. In addition‚ an albumin and pre-albumin level should be obtained to look at the amount of protein in your body. If levels such as glucose or white blood cells are high healing will be delayed. On the other hand‚ if one’s hemoglobin and hematocrit are low‚ the wound may not receive the oxygen rich blood it needs. If the pre/albumin is low the protein needed to promote wound healing is not available (Ahmed‚ 2016). Corrective measures much be taken to correct all abnormal
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