Temperature and PH of Barley Amylase Abstract The purpose of this experiment was to find the optimal temperature and pH of barley alpha-amylase. I hypothesize that the optimal temperature would be 55 degrees Celsius and the optimal pH would be 5.5. In this experiment‚ the starch is used as a substrate to examine the optimum temperature and pH for the reaction of alpha amylase. It is known that the measuring of disappearance (absorbance) of the substrate starch with iodine using spectrophotometer
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Instructions: a) Formal writing assignment #2: The Peerless Starch Company of Blair‚ Indiana. b) Grading to be based on the CWE scoring rubric previously provided. c) Assignment must be placed on the online portfolio and must be submitted to etutoring for review. d) Read the case study below in its entirety and give it some serious thought. Then‚ in your own words‚ summarize the issues involved in this case (there are quite a few) and indicate whether Glen Baxter has a case and provide a thorough
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Title: Testing For Macromolecules Objective: Introduction Proteins are complex molecules which consists of carbon‚ oxygen‚ hydrogen and nitrogen. All proteins are made up of one or more polymers‚ known as polypeptides. Its monomers are amino acids. Protein can be divided into primary structure‚ secondary structure‚ tertiary structure and quaternary structure. It is important for all living things in the world as they form enzymes‚ antibodies and hormones. Materials and Procedures:
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enzyme amylase. Enzymes are composed of proteins and acts as a catalyst to speed up the rate of a specific chemical reaction by lowering the activation energy. Reactions do not need enzymes to occur‚ but the human body and other living organisms depend on the use of enzymes in order for biochemical reactions to happen in milliseconds. Each enzyme contains a distinct three dimensional structure which is related to its particular function. Enzymes will catalyze one specific chemical reaction since an
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Bread contains starch. The starch content in bread is derived from the starchy endosperm of wheat. Refined breads have the bran and germ removed from the wheat in order to produce softer‚ silkier flour. there is a lot more food containing starch like: Starchy foods such as potatoes‚ bread‚ cereals‚ rice and pasta should make up about a third of the food you eat. Where you can‚ choose wholegrain varieties‚ or eat potatoes with their skins on for more fibre. Starch is the most common form of
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act as a catalyst by lowering the activation energy of a reaction. Each enzyme binds closely to the substrate; this greatly increases the reaction rate of the bounded substrate. Amylase enzyme‚ just like any other enzyme‚ has an optimum PH and temperature range in which it is most active‚ and in which the substrate binds most easily. The purpose of this experiment was to determine (1) the reaction rate of an amylase enzyme in starch and (2) the environmental factors that can affect the enzymatic
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Amylase And Its Functionality At Various Ph Level Abstract Enzymes can be denatured at various ph levels in which then affects the shape of the enzymes structure and reactivity. The altering of shape causes substrates to not bind in the active site (Scott Freeman‚ Micheal Harrington‚ Joan. C Sharp‚ 2009). Amylase is used as a catalytic enzyme to determine the time period to convert starch into glucose monomers and transport into the bloodstream at different ph levels
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Introduction Starch is composed of α-amylose and amylopectin. The structure of α-amylose consists of long polymer chains of glucose units connected by an α (1-4) linkage. Amylopectin consists mainly of α (1-4) linked glucose residues but is a branched molecule with α (1-6) branch points every 24 to 30 glucose residues on average. As a result of the bond angles in the α (1-4) linkage‚ amylose actually forms a spiral much like a coiled spring. Amylose is responsible for the formation of a deep blue
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Property of Starch The experiment obtained Starch to have a bulk density of 0.48g/ml‚ i.e. 0.48g of Starch occupies 1ml of water. This value represented the density of starch incorporating the voids. The tapped density was found to be 0.60g/ml. This value represented the density of starch excluding the voids and intra- particle pores greater than molecular and atomic dimensions in the crystal lattice. This simple means‚ by reducing the number of intermolecular spaces between the starch particles‚
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Introduction This practical experiment was performed in an attempt to observe how enzymes (starch phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate;
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