The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
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According to the data from both the lab group and the class average‚ there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g‚ this gained 2.2g‚ an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in
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In the unknown identification labs‚ we have identified our unknown as Pseudomonas aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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Maryely Ramirez Professor Theresa Sterner Bio Lab 105 04 November 2013 The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is
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A. Avril Crayfish Lab Report November 9‚ 2012 Dr. Marvin Results: Figure 1. Firing Rate of Tonic Receptor in Response to Stretch. The correlation between Firing Rate and Stretch of the slow adapting crayfish receptor for four different sets of data is represented in this figure. The recordings are taken at stretches of 2‚ 4‚ 6‚ 8‚ and 10 mm of the crayfish tail. The best fit lines for the different sets of data are as follows: Ali and Emily- Linear best fit line‚ Dave and Laura- Exponential
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January 20‚ 2013 Introduction and Purpose Hypothesis – When changing the level of precipitation which changes the seeds to hard large seeds the finches with the smaller beaks with become a smaller population when changing the precipitation which changes the seeds to smaller soft seeds will increase the population no matter what size of the beaks. This experiment is being done to study natural selection‚ speciation‚ and human evolution. Although no humans are being observed in this lab the concept of
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Purpose Determine the acceleration in a quick sprint. Question What would the participant’s acceleration be if he/she sprints forward in a positive direction? Hypothesis/Prediction When a person sprints forward‚ it means he/she speeds up. Consequently‚ the acceleration should be positive. When the velocity accelerates at a constant rate‚ the acceleration should remain constant. Therefore‚ if the participant is moving toward a positive direction and the speed increases‚ then the acceleration
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