The Effects of pH‚ Temperature‚ Enzyme‚ and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed by amino acids chains. Enzymes attract and fit substrate molecules to an active site. The active site binds the substrate molecules covalently to enzyme forming an enzyme-substrate complex‚ which catalyzes
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Enzyme Inhibition Many drugs exert their action by inhibition of an enzyme activity in the body. If the activity of an enzyme is vital to the cell or organism‚ then inhibition may lead to death of the cell or organism. It is now possible to design new drugs which are enzyme inhibitors once a target enzyme has been identified. Types of Inhibitors A) Reversible Inhibitors: The effect of the inhibitor is instantaneous‚ and it can be removed from the enzyme by dialysis so that the enzyme activity
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Computer Enzyme Action: Testing Catalase Activity 6A H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great
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Explain the use of enzymes in food production by means of examples. You must include the example of lactase. Enzymes are proteins that speed up the rate of chemical reactions (up to a million times) in living organisms. Acting as catalysts they are not consumed nor altered in the process of converting the specific set of reactants into specific products. In food production‚ enzymes are greatly appreciated by their accelerated effect in biochemical processes and are mostly used in what we
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Enzyme concentrations Hypothesis The higher the concentration of protease enzyme the higher the rate of breakdown of the egg white. This is because egg white is mainly formed of collagen‚ which is a protein‚ which is what the enzyme protease breaks down. The more enzyme there is the more enzyme substrate complexes are formed which break down peptide links between the monomers of the protein accelerating the amount of protein broken down in a space of time. How the results will be obtained
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constants (Km and Vmax) of extracellular purified pectinase. 3.8.3. Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in
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Investigating the effect of different environmental factors on the reaction of liver and hydrogen peroxide Table of Contents 1 Design 3 1.1 Variables 3 1.2 Safety and Environment 3 2 Data Collection and Analysis 3 2.1 Collected Raw Data 3 2.2 Qualitative data 5 2.3 Processed Data 5 2.4 Graph on test tube 2 5 2.5 Graph on test tube 3 6 2.6 Errors 6 3. Conclusion and Evaluation 6 3.1. Conclusion 6 3.2. Evaluation 7 3.2.1. Random Errors 7 3.2.2. Systematic Errors 7 3.3. Improvements
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Investigation Aim: The aim of the investigation was to investigate the effect of substrate concentration Hydrogen Peroxide H O (in %) on the rate of reaction of the enzyme catalase (in 1/mean time). Prediction: As the substrate concentration (hydrogen peroxide) in % increases the rate of reaction in 1/mean rate increases until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached‚ then adding extra substrate will make no difference. The
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Title: The Effect of Adjusted Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase. Hypothesis: Null Hypothesis: If the concentration of the Hydrogen Peroxide is changed then there would be no change in the reaction rate. Alternate Hypothesis 1: I there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will increase. Alternate Hypothesis 2: If there is an increase in concentration in concentration of Hydrogen
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Investigating Marketing Unit 3 Applied Business Introduction This piece of coursework is for my Unit 3 Applied Business. The coursework involves investigating marketing within a chosen business. The business I have chosen to investigate is Kitchen‚ a small business located in Snaith. The aim of my coursework is to evaluate the marketing strategy used by Kitchen to sell bakery and deli produce and also to operate a café. To investigate my chosen business I will give a brief background to Kitchen
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