Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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Enzymes are naturally occurring biological catalysts that are extremely efficient and specific. Enzymes accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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Q1: To study the effect of camouflage on a predator-prey relationship [F4-Chapter 8] Q2: To study the relationship between the concentration of CO2 and the rate of photosynthesis [F4-Chapter 6] 2004 Q1; To determine the % of vitamin C in fruit juices using DCPIP solution [F4-Chapter 6] Q2: To estimate the size of the bird population [F4-Chapter 8] 2005 Q1: To determine and compare the energy content in white bread and peanuts [F4-Chapter 6] Q2: To determine the concentration of the solution
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Digestion and Enzymes – APP. Hypothesis: The enzyme‚ Amylase which is used to break down carbohydrates will work the best when heated at 40°C. Also‚ as the temperature increases the reaction rate of amylase increases too. However‚ the reaction rate of amylase will start decreasing when the temperature reaches the enzyme’s optimal temperature. Many enzymes are specific for a certain substrate. For example‚ lipase is a specific enzyme for fat substrates and protease‚ a specific enzyme for protein
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The Rate of Reaction that Enzyme Concentration‚ pH‚ and Temperatures Have on the Amylase Enzymes Color Disappearance Abstract: Compare reaction rates of the concentrations‚ pH’s‚ and temperatures of the enzyme Amylase. At what concentrates do the substrate molecules collide with each other‚ making the reaction possible? At what pH levels do the 3D molecular structures change breaking the H-bond and/or denaturize? At what temperatures do the collisions of the substrate molecules happen
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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The Effect of Salt Concentration on Osmosis in Potato Cells We should first explain what Osmosis is. It’s a passage of water from a region of high water concentration through membrane to a region of low water. Semi membrane is a very thin layer of material‚ which allows some things to pass through them but prevent other things from passing through. The cells allow small molecules like Oxygen‚ water‚ Carbon Dioxide‚ Ammonia. But will not allow larger molecules like Sucrose‚ Starch‚ or protein‚ etc
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