How Environment Can Effect Enzymes Introduction: In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy‚ more of the substrate is able to participate in the reaction‚ speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react
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Enzyme Lab: Peter Kuetzing – 10/4/2012 – F Block 1. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? When more enzymes is added the rate of reaction speeds up. In this case‚ H2O2 will produce more O2‚ in return the kpa/min will go up. 2. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? Predict what the rate would be for 5 drops. I think that the rate of reaction will slightly increase from
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STANDARD GRADE BIOLOGY CREDIT LEVEL REVISION QUESTIONS INVESTIGATING CELLS 1. In an investigation‚ three 25 g samples of sultanas were put into separate beakers of distilled water‚ as shown below: After 24 hours‚ the sultanas were removed from the water‚ blotted on filter paper and reweighed. The results are shown in the table: |Sample |Mass after 24 hrs (g) |Percentage change in mass | |1
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Aim: In this investigation I will be measuring the effects of temperature on the membrane permeability of beetroot. I will be measuring the amount of anthocyanin that will diffuse out of the beetroot. The way in which I will measure the anthocyanin is to check the light absorbency of the solution using a colorimeter. The higher the reading on colorimeter the more anthocyanin present in the solution To find out the permeability of the beetroot membrane I will firstly cut out cylinders of beetroot
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Enzymes are catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site‚ an area in which a material to be acted on by the enzyme‚ called the substrate‚ will fit. The temperature‚pH‚ the concentration of enzyme‚ and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction. The
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third reaction involves molecular iodine becoming a dark blue starch;I2(aq) + starch blue-black complex3. These three reactions react in a sequence. In any reaction the concentration is exponentially related to the rate of the reaction. The rate of the reaction is dependent on potassium iodate4. Therefore as the concentration increases‚ the rate of the reaction also increases. Since the rate of the reaction is inversely proportional to time‚ as the
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Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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Enzymes Lecture outlines •Catalysis profile •Activation energy & its •Enzyme & substrate substrates •How enzymes bind to •Lock & Key model •Induced-fit model •Enzyme assay Lecture outcomes • At the end of this lecture‚ students are able to: • Define the catalyst • Understand how enzymes work as catalysts‚ the concept of activation energy and enzymes-substrate binding • Explain different theories of the relation between enzymes and substrates Catalysis • It is probably
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Design your own experiment: Effect of pH on enzymatic activity Background Information: Trypsin is a protease which conducts hydrolysis forming peptides. It is an enzyme which is secreted by the pancreas into the small intestine and works best in an alkaline environment. Egg white is used in this practical as it is found to contain the protein/ enzyme trypsin. pH is the measure of the amount of H+ ions in a solution‚ these ions affect the shape of the enzyme. Hypothesis: That as pH increases
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Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in the cells of the body
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