Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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The halides in unknown solution were identified as iodide and chloride based on their experimental E°(Ag/AgX) values‚ -0.16 ±0.01V and 0.22 ±0.02V respectively. These results were both accurate with percent error of 5.96% for iodine and 0.9% for chloride. The literature value of E°(Ag/AgI) and E°(Ag/AgCl) were -0.151V and 0.222V respectively (1). In addition‚ the literature vales lay within the 95% confidence interval for both halides therefore the results were accurate. These results were precise
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Effect of Nature of Reactants to the reaction rate. B. Effect of Temperature to the reaction C. Effect of Concentration to the Reaction Rate D. Effect of Catalyst to the Reaction Rate E. Chromate-Dichromate Equilibrium F. Thiocyanatoiron (III) Complex Ion Equilibrium G. Weak Acid Equilibrium (Ionization of Acetic Acid) H. Weak Base Equilibrium Ionization of Ammonia I. Saturated Salt (Sodium Chloride) Equilibrium On part (A) we are to observe which reaction rate is faster‚ and doing the experiment
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Kinetic theory (or the kinetic or kinetic-molecular theory of gases) is the theory that HYPERLINK http//en.wikipedia.org/wiki/Gasgases are made up of a large number of small particles (HYPERLINK http//en.wikipedia.org/wiki/Atomatoms or HYPERLINK http//en.wikipedia.org/wiki/Moleculemolecules)‚ all of which are in constant‚ HYPERLINK http//en.wikipedia.org/wiki/Randomnessrandom HYPERLINK http//en.wikipedia.org/wiki/Motion_(physics)motion. The rapidly moving particles constantly collide with each other
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KINETIC ENERGY Objects have energy because of their motion; this energy is called kinetic energy. Kinetic energy of the objects having mass m and velocity v can be calculated with the formula given below; K=1/2mv² Kinetic energy is a scalar quantity; it does not have a direction. Unlike velocity‚ acceleration‚ force‚ and momentum‚ the kinetic energy of an object is completely described by magnitude alone. Like work and potential energy‚ the standard metric unit of measurement for kinetic energy
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The electron transfer reaction between hexacyanoferrate (III) and sodium borohydride resulted in the formation of hexacyanoferrate (II) ion and dihydrogen borate ion which was strongly catalyzed by AuNPs. The redox reaction is described as BH4- + 8 [Fe (CN)6]3- + 3H2O 8 [Fe(CN)6]4- + H2 BO3- + 8H+ The advantage of hexacyanoferrate ion for this redox study is that both oxidation states of iron (+2 and +3) are quite stable with respect to dissociation and hydrolysis
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Subject: Biology LESSON PLAN Lesson: Chemical Reactions + Catabolism/Anabolism Number of Meetings: 1 Behavioral Objectives: At the end of the lesson‚ students should be able to: a.) Relate chemical reactions with metabolism b.) Identify the major components of a chemical reaction c.) Describe at least one everyday example of an endergonic and exergonic reaction d.) Relate the breakdown and formations of organic molecules to catabolic or anabolic reactions Reference: *
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The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor constant for
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BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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There are various types of essay that every High School‚ College and University students gets to encounter in the course of the academic program. Essays are typical writing assignments that require a writer to use personal input in order to convey an idea‚ present/defend an argument or simply get across some information to the audience. There are numerous types of essays that our essay writers can assist you with. 5 paragraph essays 5 paragraph essay is the classic. It is strictly structured
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