Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in the cells of the
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BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Enzymes are naturally occurring biological catalysts that are extremely efficient and specific. Enzymes accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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Design: Investigation of Fermentation Introduction Introduction Yeast (Saccharomyces)is a single-celled microorganism in the Fungi family. It anaerobically respires sugars to produce ATP‚ as well as the waste products ethanol and carbon dioxide gas. This process is known as fermentation. There are various factors that affect the rate at which yeast respires. Aim To investigate the effect of concentration of table salt (sodium chloride) on the rate of fermentation of sucrose using yeast‚ measured
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Enzymes Lecture outlines •Catalysis profile •Activation energy & its •Enzyme & substrate substrates •How enzymes bind to •Lock & Key model •Induced-fit model •Enzyme assay Lecture outcomes • At the end of this lecture‚ students are able to: • Define the catalyst • Understand how enzymes work as catalysts‚ the concept of activation energy and enzymes-substrate binding • Explain different theories of the relation between enzymes and substrates Catalysis • It is probably
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I. Preparation A. Choosing 1. You should choose the fruits that you like. a. For example‚ if you desire to gain more different fruits enzymes or make the glass bottle more colorful‚ you can choose four or even more kind of fruits. There is no limitation. But‚ you must include one fruit‚ lemon. It helps to kill the bacteria of the drink. B. Composing 1. The ratio of components should be 3‚ 1. It means 3 parts of fruits‚ 1 part of sugar. You can follow this ratio to determine the amount of fruit
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Enzyme Catalysis Abstract: Catalysis is an enzyme that decomposes hydrogen peroxide into oxygen and water. In this lab we will conduct a series of experiments to determine the affects of; pH‚ temperature‚ and concentration change on such catalysis. After completing the experiments we determined that catalase works most efficiently when in a 27°c‚ 50% (5 ml of catalase – 5ml of water)‚ and a normal body pH of 7°. By: Patrick Jawien Course Code: SBI 4U Performance Date: 25 September 2012
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TASK 4: Explain the structure of the ileum and the ultrastructure of the epithelium in relation to its secretory and absorptive functions. (a) Explain the characteristics of enzymes. (b) Explain the lock and key theory of enzyme action. (c) Locate and explain the role of important digestive enzymes. TASK 5: Enzymes are involved in anabolic and catabolic reactions: a) Define the terms anabolic and catabolic reactions b) Give 3 examples of anabolic and 3 examples of catabolic reactions
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The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
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