Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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Spectrophotometric Determination of the Equilibrium Constant of a Reaction DEPARTMENT OF MATERIALS‚ METALLURGICAL AND MINING ENGINEERING‚ COLLEGE OF ENGINEERING July 26‚ 2012 ------------------------------------------------- ABSTRACT ------------------------------------------------- Before proceeding to test the next solution‚ we must first clean the cuvette‚ using similar steps earlier. First‚ we rinse with water‚ then with the solution‚ and then we fill it up and test
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Chemistry Internal Assessment 2011 Candy Au Introduction The reaction between zinc and copper (II) sulphate is endothermic. If an excess of zinc powder is added to a measured amount of aqueous copper (II) sulphate in a calorimeter and the temperature change is recorded over a period of time‚ the enthalpy change of the reaction can be experimentally determined. Assumption 1. Mass of H2O in 100cm3 of CuSO4 solution is 100g 2. Specific heat of solution is 4.18 kJ kg-1 K-1 which is the
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The graph for run 1‚ which plotted ln(Abs at 595nm) versus time‚ a first order reaction‚ with an R^2 value of 0.99621 was the most linear. Therefore‚ crystal violet is a first order reaction. The observed rate constant from run 2‚ as shown in figure 4‚ was significantly lower then the observed rate constant from run 1‚ shown in figure 1. Therefore‚ the R^2 for the first order reaction for run 2‚ 0.946418‚ represented in figure 4‚ which is also extremely low‚ was not taken into consideration for calculations
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In this experiment‚ a coffee cup calorimeter was used to measure the temperature readings of a neutralization reaction and magnesium oxide‚ MgO. Calorimetry is used to measure amounts of heat transferred to or from a substance.2 The difference of temperatures was used to calculate the heat energy given off by each sub-reaction. These values were solved by using Hess’s Law which determined the overall enthalpy changes of the neutralization reaction and MgO formation. Hess’s Law states that the heat
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According to the evidence derived from our lab‚ we have determined that Unknown A‚ B‚ C‚ and D are to be identified as the Sprite‚ burger‚ fries‚ and bun‚ respectively. Each Unknown provided evidence that we eventually used to match to each element of the meal. Along with the lab data‚ our prior knowledge also led us to our results. We were able to determine that Unknown A is the Sprite due to the fact that every test was negative except for simple sugars‚ a macromolecule that the majority
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INDEPENDENT VARIABLES: • The number of carbon atoms in the molecule. DEPENDENT VARIABLES: • Mass of alcohol burnt CONTROLLED VARIABLES: • The length of the wick coming from the spirit burner • Distance of the wick from the bottom of the aluminium can • Temperature change of the water in the aluminium can • Can used as calorimeter • Mass of water in calorimeter • The room temperature (environment) It is important that the controlled variables are all kept the same because it makes the
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"The difference between the impossible and the possible lies in a man’s determination." Tommy Lasorda As fast as light… Winners don’t wait for chances‚ they take them - Unknown Sports serve society by providing vivid examples of excellence. George F. Will (1941 - ) Sports do not build character. They reveal it. It’s not so important who starts the game but who finishes it. John Wooden (1910 - ) Desire is the most important factor in the success of any athlete. willie shoemaker "Winning
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Lab 2: Spectrophotometric Determination of Iron In Vitamin Tablets Objectives: - To determine the amount of iron in a generic vitamin tablet on a per tablet (in grams) basis. - To prepare standards using a pre-made iron solution to help determine the iron content in our generic vitamin tablets - Determine absorbances of our vitamin tablets and standards using the spectrophotometer Method: 1. Mass three vitamin tablets that contain iron 2. Place each vitamin in a 125-mL Erlenmeyer flask
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Determination of an Unknown Concentration of Potassium Manganate using Colorimetry Introduction The purpose of this experiment is to conclude the concentration of an unknown solution using colorimetry to plot a calibration curve. Colorimetry measures the absorption of light at wavelengths within the visible region of the electromagnetic spectrum. It can be used to identify the unknown concentration of a solution in reference to a solution of a known concentration. When a solution appears
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