Evaluation of the phytochemical composition and the anti-proliferative potential of seed extract of Dicerocaryum senecioides on Jurkat T cell lines By Humbulani Ronald Tshilongamulenzhe A Dissertation submitted to the Faculty of Science and Agriculture‚ Department of Biochemistry‚ Microbiology and Biotechnology‚ School of Molecular and Life Sciences in accordance with the requirements of the University of Limpopo for honours for the degree Bachelor of Science in Biochemistry UNIVERSITY OF LIMPOPO
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Discussion and Interpretation of Results The addition of HBr to 1-hexene created a 2-bromohexane‚ as indicated from the ethanol silver nitrate test and sodium iodide/acetone test. The observations when the product was subjected to these tests were similar to what were observed with the 2° bromide compound. When subjected to the silver nitrate test‚ the product produced a precipitate at the same rate of the 2° bromide compound‚ and the product produced a hue of yellow that was also similar to the
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ions with dextrose. The materials that we used were acetone‚ 5 mL of 1.5 M ammonium nitrate solution (NH4NO3)‚ 5 mL of 0.5 silver nitrate solution (AgNO3)‚ 10 mL of 5% dextrose solution (C6H12O6)‚ 10mL of 10% sodium hydroxide solution (NaOH)‚ a 50 mL beaker‚ a 250 mL Florence flask with a rubber stopper‚ a 10 mL graduated cylinder‚ a waste beaker‚ and distilled water. The first thing we did was rinsed the beaker‚ cylinder‚ and flask with acetone. To start‚ we poured the 10 mL of 5% dextrose solution
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The purpose of Module 11A was to test for the presence or absence of a particular set of functional groups through the use of wet chemical tests. In this manner‚ Unknown A which was a colorless solution‚ was first tested with 2‚4-DNP which after mixing for a few seconds formed a bright yellow precipitate. Although this confirms the presence of either a ketone or aldehyde group‚ one simple chemical test does not completely specify the presence or absence of other functional groups. Therefore‚ a second
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in 100ml of trisodium citrate solution and boiled the solution for 15min. the solution was cooled and For the conjugation of curcumin 50mg of curcumin dissolved in 25ml of acetone and this solution added to above solution and reaction mixture stirred continue for 3hr at 60ºC without reflux condenser for complete removal of acetone from the
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displayed through Figure 2‚ where the retention rate is 1.275 minutes and its peak signal is 1.4 mV. The range for methanol due to its similar voltage and retention time‚ when comparing Figure 2a and 2b. Because these chemical mixtures contain acetone‚ methanol‚ and 2-butanone‚ the simplest way to determine the volatility of these chemicals is through gas
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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Solubility‚ Crystallization and Melting Point Determination Post-Lab Discussion Guidelines: Part 2A and 2C: Draw tables (as in textbook) showing which combinations were soluble/miscible and which were insoluble/immiscible. Explain why this is so. Why are certain chemicals soluble/miscible when others are not? Part 3A: Calculate % recovery (this is not the same as % yield – see handout from the first day of lab if you’re confused)‚ and determine melting point of your product. Discuss % recovery
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bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink Microscope Clothes pin Bunsen burner Methods: 1. Obtain your slide and draw two circles where your bacteria will go. 2. Connect you Bunsen burner and run your inoculation loop through the flame to sterilize it
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because temperature affects the structure of the protein. Also‚ ethanol is added to remove some impurities because ethanol here acts as precipitating agent. Later on‚ the solution is once again centrifuged. The crude is weighed and is washed with acetone to fasten drying. It is then prepared for characterization. B. Albumin from egg Egg white is acquired because it is where albumin is present. It is gently stirred to prevent denaturation and to mix the enzymes present in it. Later on‚ it is
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