Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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Solubility‚ Crystallization and Melting Point Determination Post-Lab Discussion Guidelines: Part 2A and 2C: Draw tables (as in textbook) showing which combinations were soluble/miscible and which were insoluble/immiscible. Explain why this is so. Why are certain chemicals soluble/miscible when others are not? Part 3A: Calculate % recovery (this is not the same as % yield – see handout from the first day of lab if you’re confused)‚ and determine melting point of your product. Discuss % recovery
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because temperature affects the structure of the protein. Also‚ ethanol is added to remove some impurities because ethanol here acts as precipitating agent. Later on‚ the solution is once again centrifuged. The crude is weighed and is washed with acetone to fasten drying. It is then prepared for characterization. B. Albumin from egg Egg white is acquired because it is where albumin is present. It is gently stirred to prevent denaturation and to mix the enzymes present in it. Later on‚ it is
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bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink Microscope Clothes pin Bunsen burner Methods: 1. Obtain your slide and draw two circles where your bacteria will go. 2. Connect you Bunsen burner and run your inoculation loop through the flame to sterilize it
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Alkyl Halides Alkyl halides are a class of compounds where a halogen atom or atoms are bound to an sp3 orbital of an alkyl group. CHCl3 (Chloroform: organic solvent) CF2Cl2 (Freon-12: refrigerant CFC) CF3CHClBr (Halothane: anesthetic) Halogen atoms are more electronegative than carbon atoms‚ and so the C-Hal bond is polarized. H H μ C + C-l δ δ H The C-X bond is polarized in such a way that there is partial positive charge on the carbon and partial negative charge on the halogen. Dipole moment
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CHE656 Computer Applications for Chemical Engineering Practice Process Simulation with ASPEN PLUS Exercise Problems Version 2012 Prepared by Dr. Hong-ming Ku King Mongkut’s University of Technology Thonburi Chemical Engineering Department Chemical Engineering Practice School © May 2004-2012 – Use with Permission of the Author Only 1 Section 1 Elementary Modules‚ Mass Balances‚ and Degree of Freedom Analysis 2 1. Mass Balances and Constraints with Elementary Modules
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I (insulin-dependent) diabetes mellitus was found in a coma. His blood glucose was high‚ as well as his urine glucose‚ urine ketones‚ and serum ketones. His serum bicarbonate was <12 mEq/L. His respiration was exaggerated and his breath had an acetone odor. His blood pressure was 90/60 and his pulse weak and rapid (120). ------------------------------------------------- Top of Form 1. Define noncompliant.: a nursing diagnosis approved by the North American Nursing Diagnosis Association‚ defined
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Phenol‚ also known as carbolic acid‚ is an aromatic organic compound with the molecular formula C6H5OH. It is a white crystalline solid that is volatile. The molecule consists of a phenyl group (-C6H5) bonded to a hydroxyl group (-OH). It is mildly acidic‚ but requires careful handling due to its propensity to cause chemical burns. Phenol was first extracted from coal tar‚ but today is produced on a large scale (about 7 billion kg/year) from petroleum. It is an important industrial commodity as a
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Hypothesis. If pigment from euonymus alatus (burning bush) leaves is extracted‚ then carotenes and xanthophylls will appear when a solvent‚ made of 9 parts petroleum ether and 1 part acetone‚ goes up Whatman #1 filter paper Purpose. The purpose of this experiment was to demonstrate the presence of carotenes‚ xanthophylls‚ and chlorophylls in leaves. Methods and Materials. A piece of Whatman #1 filter paper was cut to fit into an ignition tube. One end was cut to form a V‚ and the sides of
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nExperiment C: Kinetics of the Hydrolysis of T-butyl Chloride Post-Lab Ch202 Postlab Assignment Nov 5/2014 L10 Radwan Ahmed 130770850 1. What was green about this experiment was that the solvents used (acetone and water)‚ and even the tert butyl chloride were fairly safe to handle with respect to the other chemicals used in the lab. This is why gloves were not worn‚ and one reason why this experiment follows GCP # 3 where chemical products should be designed to preserve efficacy of the function
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