Introduction Identification and classification of bacteria is important to make easier manipulation on the bacteria for various purposes such as for medical‚ research‚ developmental‚ and biotechnicalfieldsThe cell wall is the basis for classification of bacteria according to the Gram stain. Accordingto the chemical make up of bacterial cell wall‚ a staining procedure‚ Gram stain‚ helps usclassify bacteria into two subgroups‚ The cell wall can stain either positive or negative‚depending on its chemistry
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RECRYSTALLIZATION AND MELTING POINT DETERMINATION OF BENZOIC ACID ANSWERS TO QUESTIONS 1. How does the use of fluted filter paper hasten filtration? Why is it advisable to place a small piece of wire between the funnel and the mouth of the flask during hot filtration? Fluted filter paper is effective in preventing crystal formation in the filter paper. It is also often used in filtering hot saturated solution used during crystallization. One major advantage of a fluted filter is that it increases
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Lipids Biochemistry November 13‚ 2013 Lipids Lipids are families of biomolecules including fats and oils‚ waxes‚ steroids ‚ cholesterol and fat soluble vitamins. All lipids are naturally occurring compounds varying greatly in structure but share the common feature of being soluble in non polar solvents such as chloroform and ether but not in water. Lipids consist mainly of carbon and hydrogen atoms linked by non polar covalent
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Alkyl Halides Alkyl halides are a class of compounds where a halogen atom or atoms are bound to an sp3 orbital of an alkyl group. CHCl3 (Chloroform: organic solvent) CF2Cl2 (Freon-12: refrigerant CFC) CF3CHClBr (Halothane: anesthetic) Halogen atoms are more electronegative than carbon atoms‚ and so the C-Hal bond is polarized. H H μ C + C-l δ δ H The C-X bond is polarized in such a way that there is partial positive charge on the carbon and partial negative charge on the halogen. Dipole moment
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Bioenergetics Photosynthesis & Respiration Laboratory Report Exercise 6 PBIO101 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Gina Dedeles Minda Dimaano-Kho Group 5 Felicita‚ Haniel Paulo‚ Gisselle Mildred V. Aniseta‚ Carmelus*Absent but present
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Potato juice! 7. Sucrose juice! 8. Glucose juice! 9. Distilled water! 10. Reducing-sugar solution! 11. Starch solution! 12. Sodium hydroxide (NaOH)! 13. Egg albumen! 14. Honey! 15. Amino acid solution! 16. Protein solution! 17. Water! 18. Acetone! 19. Vegetable Oil! 20. Salad oil! 21. Known lipid solution ! 22. Sudan IV! 23. Brown wrapping paper or brown paper bag! 24. Eyedropper! 25. Pipette! 26. Test tube holder! ! ! ! METHODS ! ! Benedict’s test for reducing sugars
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Adol Condensation Introduction: This reaction is carried out by adding benzaldehyde and acetone into a flask. The product created is a 1‚5-diphenyl-1‚4-pentdiene-3-one‚ which includes two double bonds‚ and two benzyl ring functional groups. This is a dehydration reaction that occurs twice in order to form the diene. After obtaining the product‚ via vacuum filtration‚ it will be recrystallized and then analyzed for purity by determining both products’ melting point. The two products will be
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workshop‚ to produce the powder. The process was similar to that of making kajal. The researchers used two earthen pots—one to keep wood wool and the other to cover it. The wood wool soot deposited on the upper pot was scraped out and washed with acetone. The washed soot was then air dried and oxidised with nitric acid to obtain wsCNPs. The nanoparticle was tested on three types of mosquitoes–Anopheles‚ Aedes and Culex. To monitor the growth and life cycle‚ optical fluorescence microscope was used
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procured from Agilent Technologies (USA). HPLC grade acetonitrile‚ acetone and ethyl acetate were supplied by Merck (Mumbai‚ India). Analytical grade sodium chloride (NaCl) and anhydrous magnesium sulfate (MgSO4) was obtained from Merck. Anhydrous magnesium sulfate was activated in a muffle furnace at 400 ºC for 6 hr before to use and kept in desiccators. Individual standard stock solutions of 1000 µg/mL were prepared in acetone in a class A grade volumetric flask of 25 ml capacity by dissolving
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