MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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Health and Safe Legislation and Regulation You are required to research two different engineering environments that you have come knowledge of. It may be best if one is an electrical environment and one a mechanical environment. For each of these environments: • Identify a minimum of four different sets of regulations that would be relevant • Describe a minimum of three key features of each of the sets of regulations you have chosen. Mechanical Provisional and Use of
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LABORATORY REPORT FOR BIO411 Experiment 5 : Cellular Respiration Experiment 6 : Photosynthesis Title: Cellular Respiration Objective: To observe and determine cellular respiration in yeast/onion cells. * Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing
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We recorded our findings on the table labeled table 1. Table 1 shows the transmittance at each wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used; with the help of acetone we saw the spectrum and the differences of the different pigments. Percentage transmittance in Table 1 show that the lower the number‚ the more dense that solution is at that specific wavelength. As the numbers get higher‚ the less dense it is and
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as recrystallizing solvent: 2 mL 6. Mass of purified salicylic acid: 0.134 g 7. Percent yield of purified salicylic acid from reaction: 63.5% 8. Melting point of purified product: 158-160 oC 9. Name of NMR solvent used and its chemical shifts: Acetone-d6‚ the chemical shifts are 29.9 and 206.7 for 13C Questions: 1. 2. The phenol group of methyl salicylate is a weak acid‚ therefore it reacts very swiftly with a strong base‚ in this case NaOH‚ to form a sodium
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Materials list Test tubes‚ Water‚ small beacon‚ Pipette‚ Stand‚ Mortar‚ green leaves‚ Filter paper‚ capillary tube‚ funnel‚ silicon dioxide‚ calcium carbonate‚ acetone‚ solvent. Method 1) To extract pigments from green Leaves - Weigh 5g green Leaves‚ cut into pieces‚ and put in mortar. - Add silicon dioxide + calcium carbonate + 5ml acetone and grind (Try not to inhale fumes) - Pour the
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Ketones in insulin resistant state 1. How is glucose normally maintained in the post absorptive state? 2. Why is muscle glycogen not useful for blood glucose 3. Why may this situation have evolved 4. Under what circumstances do you think substantial gluconeogenesis will occur 5. What is necessary for the production of ketones 6. What will reduce acetyl CoA oxidation 7. When is oxaloacetate availability impaired 8. When is the regulation of gluconeogenesis disrupted
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Title: Freezing and Melting of Paradichlorobenzene Purpose: Melting‚ freezing‚ and boiling points are properties that can easily be determined and help in identifying a substance. These properties can be found in chemical literature ad reference tables‚ and can be used to distinguish a pure substance from many other pure substances. In this lab you will use the pure substance Paradichlorobenzene and determine its melting an freezing points by warming and cooling a sample of the substance
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Technology Madras Engineering Chemistry III Prof. K. M. Muraleedharan Reaction Electrophile Generation of electrophiles Cl2 + FeCl3 Cl3Fe Cl Cl First step Chlorination Cl+ bromination Br+ Br2 + FeBr3 Br3Fe Br Br Iodination
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spectrophotometric method of assay. After storage of these cells at 4 C reproducible results in replicate tests were not obtained. Desiccation of the phenol-killed cells with ’acetone did not provide a satisfactory cell preparation. Such cells gave variable results and were difficult to resuspend properly. It was assumed ’that ’ acetone influenced the ’sensitivity of M. lysodeikticus by reason of its fat
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