ABSTRACT Enzymes are highly specific and can distinguish isomers of the same molecule. The enzyme invertase specifically catalyzes the reaction of the conversion of sucrose to its individual carbohydrates glucose and fructose. It does not catalyse the reaction of maltose to 2 glucose or lactose to galactose. In this experiment‚ titrimetric and spectrophotometric methods were used to determine the specificity of invertase by determining the amount of glucose converted from the given disaccharides
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and you add Bieuret reagent into each‚ then the gelatin will change color. Hypothesis F1: If you add Benedict’s solution to the urine sample and boil it‚ then it will change color‚ indicating that glucose is present. Hypothesis F2: If you add iodine to the urine sample‚ then it will turn color‚ stating that there is a starch present. Hypothesis F3: If you add Biuret’s solution to the patient’s urine sample‚ then it will change color‚ meaning a protein‚ or child‚ is present. Hypothesis F4: If
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Diffusion and Osmosis NGSSS: SC.912.L.14.2 Relate structure to function for the components of plant and animal cells. Explain the role of cell membranes as a highly selective barrier (passive and active transport). SC.912.L.14.3 Compare and contrast the general structures of plant and animal cells. Compare and contrast the general structures of prokaryotic and eukaryotic cells. AA Background: (Source: www.explorelearning.com) Diffusion is the process in which there is a net movement of
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Then you titrate the iodine remaining with sodium thiosulfate solution using starch indicator. Because you know how much iodine was produced from iodate(V) and iodide and you have found by titration how much is left over you can calculate how much iodine must have reacted with the ascorbic acid. First you will need to analyse a solution of ascorbic acid with a known composition. By doing
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certain tests to determine the presence of macromolecules. In doing the lab‚ one was able to determine the characteristics of the given solutions‚ containing different macromolecules‚ whilst doing the multiple tests. The tests performed were‚ iodine testing for starch and glycogen‚ Benedict’s test for reducing sugars‚ and the Biuret’s test for protein. Depending on the test performed‚ certain results acquired‚ clearly identified the macromolecules present in each solution. With the aid of the
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the salt. And after the filtration the sand was left. Separation of solute between immiscible solvents * IODINE CRYSTALS + WATER + N-HEXANE the N-hexane turned to color violet. The method used to separate that mixture is Extraction. Extraction is the distribution of a solute in two immiscible solvents. The substances that separated are H2O and Iodine crystals with N-hexane. The iodine crystals have a very low solubility in water and it dissolves quickly when it combines with N-hexane and when
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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the water and put it into a test tube 10.Allow it to heat for 1 minute and see if there is any reaction in tube 11.Put starch in dialysis tube 12.Leave the substance in a beaker for 15 minutes to give it the time to diffuse 13.Add 2ml iodine to it and check if it has the same reaction as the lab report before 14.If there is time left‚ do control experiment to see if there is no reaction without the experimental substances (sucrose‚ glucose and starch) 15.If time left do negative
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AgBr(s) Any soluble bromide gives a silver bromide precipitate. Iodine (i) solid or (ii) solution A very dark solid (i) Gently heat the dark coloured solid. (ii) Test aqueous solution or solid with starch solution. (i) Gives brilliant purple vapour. (ii) A blue black colour. (i) Iodine forms a distinctive coloured vapour. (ii) Forms a blue-black complex with starch and in biology the test is used to detect starch with iodine solution. Tests for Halide Ions In test (i) the silver nitrate
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