During this experiment‚ the method of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was conducted using a negatively charged protein and pre-stained molecular weight markers. The hypothesis was that the molecular weight of N-acetyl-β-D-hexosaminidase B would be 28‚000 kDa. To confirm or reject the hypothesis‚ the molecular weight of N-acetyl-β-D-hexosaminidase B and the concentration of protein had to be determined. The electrophoresis of the protein gel were conducted using
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SYNTHESIS AND CHARACTERIZATION OF POLYANILINE/POLY (P-HYDROXYANILINE)/Fe3O4 MAGNETIC NANOCOMPOSITE M. R. SABOKTAKIN‚ A. M. MAHARRAMOV‚ M. A. RAMAZANOV Baku State University‚ Baku‚ Azarbaijan E-mail: mamed_r50@mail.ru Several composites have been studied for static dissipationand microwave absorbing materials based on polyaniline with metallic oxides. These composites which are conducting polymers have been widely used because of their lower density as well their good environmental stability as in
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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transportation of the highly flammable gas. It is very common to see PTFE manufacturers also manufacture TFE. The polymerization process uses a very small amount of other chemicals as initiators. Various initiators can be used‚ including ammonium persulfate or disuccinic acid peroxide. The other essential ingredient of the polymerization process is water. Polymers in the natural
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enantiomers‚ the cis product‚ or a mixture of cis and trans diols. (Figure 1) In this experiment‚ you will use a common oxidizing agent‚ KMnO4. You will also use an oxidizer that you may not have discussed in lecture called Oxone ®. Oxone is a persulfate mix that reacts with acetone to produce dimethyldioxirane. Procedure: Oxone reaction Dissolve 0.40 g oxone ® in 2.0 mL of water by shaking vigorously in a sealed vial. (Caution! Be sure the vial is sealed because oxone is a strong
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Materials and methods 1. Raw materials Full ripe cactus Opuntia dillenii fruits were harvested from the orchard of Faculty of Agriculture‚ Suez Canal University‚ Ismailia Governorate‚ Egypt during December 2014. Fruits were washed and glochides and the two distal parts (top and bottom parts) were removed. The fruits were mashed in a mixer by mixing for 15 s. After that‚ the seeds were separated‚ washed and dried by hot air in a convective dryer (WT-binder‚ Type F115‚ Germany) at 50 ºC‚ air velocity
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Natalia Nelson 6661318 Environmental Studies Section: Friday @ 2:00 with Jason Linn Fracking: The Invention of the Century or Destructive New Technology? Introduction: Possible quote – someone who supports the processs. Possible story: some analogy of something that was a good‚ necessary idea but just put off the inevitable and had a negative affect than fix it. Need for energy‚ how new ideas are necessary But is fracking really the best option? Public eye‚ people here about fracking
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chalk-looking white rocks Desiccant Looks like small gray/white rocks Acetic Acid Looks like a clear liquid Solution of Sodium Thiosulfate Looks like a clear liquid Granular Zinc Looks like shredded pieces of silver Iodine Crystals Looks like small silver balls Zinc Ion and Iodine-Iodide-Triiodide ion in water Looks like a brown liquid Solid Zinc Iodide Looks like a white powder Mineral Oil Looks like a clear liquid Silver Nitrate Looks like a clear liquid Magnesium Turnings Looks like a
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Volumetric Analysis of Vitamin C by Titration The objective of this experiment is to use a redox reaction titration to accurately determine the amount of vitamin C in a sample of lemon juice‚ orange juice‚ or grapefruit juice. Chemistry of Vitamin C The chemical name for vitamin C is L-ascorbic acid. Its molecular formula is C6H8O6; its molar mass is 176.12 g/mole. Ascorbic acid is found throughout the plant and animal kingdoms‚ occurring in citrus fruits‚ hip berries (such as rose
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solution by a redox titration using iodine. Vitamin C (ascorbic acid) is a water-soluble‚ essential oxidant that plays a vital role in the protection of the body from infections and disease it is acquired from primary fruits and vegetables. With the use of different materials as natural orange juice‚ Tampico (chemical orange juice) and other solutions that contain vitamin C we will find the amount of vitamin C in each of these concentrations with the help of iodine‚ and with that data we can compare
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