Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme.
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of the initial rate of reaction. The trick‚ of course‚ is knowing when the fixed amount of product has been formed. The following examples illustrate how this can be done. Appearing blue There are a number of so called ’iodine clock’ reactions in which molecular iodine is one of the products. Probably the most famous of these is the reaction involving hydrogen peroxide and iodide
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Chemical Kinetics: The Iodine-Clock Reaction: S2O82−(aq) + 2 I−(aq) → I2(aq) + 2 SO42−(aq) To measure the rate of this reaction we must measure the rate of concentration change of one of the reactants or products. Here‚ it is convenient to carry out a clock reaction involving the product I2. To do this‚ you will include (to the reacting S2O82− and I−) i) a small (but accurately known) amount of sodium thiosulfate‚ Na2S2O3‚ and ii) some starch indicator. The added Na2S2O3 does not interfere with
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Abstract α-amylase was immobilized covalently on iron oxide magnetic nanoparticles. The synthesis of magnetic nanoparticles was done by the coprecipitation conventional method. The chemical composition and particle size of the synthesized particles was confirmed via X-ray diffraction. Tyrosine‚ Lucien and chitosan and glutaraldehyde were investigated to make a covalent binding between the iron oxide magnetic core and the immobilized enzyme. Immobilization using chitosan and glutaraldehyde show
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is most effective‚ this aim will be carried out as an iodine clock reaction. The goal of this aim is to find out what catalyst is best to make this reaction occur at the fastest rate. 3. Determine the effects of the presence of ethanol on the rate equation. It is known that ethanol effects hydrogen peroxide and therefore it has an effect on the rate equation. This aim will find out the effect of ethanol by carrying out the iodine clock reaction with and without ethanol present and the
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mmol/L | Albumin | 30 g/L | Glucose | 12 mmol/L | Amylase | 5000 U/L --- Normal Range: 60-180 U/L | Serum: Comment: The diagnosis of acute pancreatitis is based on the clinical history‚ evidence of inflammation is known usually by computerized tomography (CT scan) and the finding of a high serum (or sometimes urinary) amylase activity. It is effectively a diagnosis of exclusion: the finding of a very high serum amylase activity is very suggestive but is not on its own diagnostic‚ as
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The Iodine Clock Investigation Introduction This is an investigation into the rate of a reaction and the factors that contribute to how fast a reaction will take place. Through the recording and analysis of raw data‚ this investigation also allows us to apply generally accepted scientific rules and to test them against results gained from accurate experimental procedures. Aim The aim of this experiment is to investigate the rate at which iodine is formed when the concentration
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Austin Peay State University Department of Chemistry CHEM 1021 BREAKING DOWN STARCH USING SALIVARY AMYLASE Caution: You will be using a Bunsen burner and glassware to create your own constant water bath. Appropriate caution should be exercised when dealing with the Bunsen burner‚ hot water‚ and glassware. Purpose: Many plants store their energy in the form of starch‚ a polysaccharide made from repeating units of the monosaccharide glucose. Our bodie
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Starch/Amylase Experiment Report Objective: The purpose of the starch/amylase experiment was to simulate and observe the process of enzyme digestion. Materials: * 1 small beaker * 2 large beakers * 2 cut pieces of soaked dialysis tubing * 2 dialysis tubing clamps or pieces of twine * 2 clean plastic pipettes * 1 bottle of Lugol’s solution * 2 glucose test strips Procedure: Begin the experiment by placing 4 full pipettes worth of cooked starch in a beaker. Then‚ use
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absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch and a buffer were
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