Trevor Smith Biol 4400 Dr. Achberger May 8‚ 2013 a. From the amino acid sequence the gene is the LacI repressor for Escherichia coli. See appendix 1 for the search result. b. The complete nucleic acid sequence of the protein with the open reading frame (ORF) indicated by red and green colorization as well as the forward and reverse primers underlined (a screenshot was taken so the sequence could be clearly displayed): c. The ORF and flanking nucleotide sequences are displayed in part “b
Free DNA Protein Gene expression
Molecular Biology 344 fall 2012 A Johnson Due 9/11/12 as hard copy at the beginning of lecture Problem Set 1 1. Look carefully at the structures of the two molecules shown below. a. What would you expect to happen if you added ddCTP (shown in a) to a DNA synthesis reaction in vitro in large excess over the concentration of deoxycytidine triphosphate (dCTP)? How will this affect the pattern of bands on the sequencing gel? The ddCTP lacks the OH‚ which is required for elongation
Premium DNA Molecular biology
Higher education buble: The higher education bubble is a hypothesis that there is a speculative boom and bust phenomenon in the field of higher education‚ and that there is the risk of an economic bubble in higher education that could have unintended consequence in the broader economy like student loan bubble that we will discuss today. Student loan bubble is a consequence of higher education bubble. According to the theory‚ while college tuition payments are rising‚ the rate of return of a college
Premium Higher education University Education
The History Bubble Gum Introduction I. Gum. It’s been around for centuries—from the ancient Greeks to the American Indians‚ everyone’s chewed it. But the best kind of gum—bubble gum!—wasn’t invented until 1928‚ when an enterprising young accountant at Fleer Gum and Candy used his spare time to experiment with different recipes. (McCarthy‚ 2010) II. Bubble gum is a type of chewing gum that is especially designed for blowing bubbles (McCarthy‚ 2010) III. Chewing bubble gum is a common
Premium Chewing gum
three dynamics that the filter bubble introduces for us and discuss the effects of each one on privacy and our internet experience. Upon reading the chapter‚ the filter bubble fundamentally alters the way we encounter ideas and information. According to the book‚ the filter bubble introduces three dynamics we’ve never dealt with before which is one‚ you are alone in it‚ second‚ the filter bubble is invincible‚ and third‚ you don’t choose to enter the filter bubble (Pariser‚ 2012). In terms of privacy
Premium Client-server World Wide Web Michael Jackson
I. Purpose The purpose of this lab is to determine the molecular mass of a volatile liquid. II. Procedure • Place a cork with a small hole in it inside of a test tube and find the weight of the two together using a sensitive balance. • Pour 0.5 mL of the unknown volatile liquid into the test tube‚ insert the cork‚ and place the tube into boiling hot water while keeping the cork above water level. As the gas evaporates‚ excess gas will be released throughout the whole in the cork. Keep the test
Premium Chemistry Water Temperature
overview Topic: “Cognitive Maps” Course: Knowledge Engineering Author: Marina Borovkova Group: FM.1 Saint-Petersburg 2011 Table of contents Introduction 3 Definition of Cognitive Mapping 4 History and Use of the Term 4 The Process of Cognitive Mapping 6 Different Types of Cognitive Map and Mapping Techniques 7 Cognitive mapping techniques 8 Causal mapping. 8 Semantic mapping 9 Concept mapping 10 Application of cognitive techniques and maps 11 Conclusion 13 Introduction
Premium Concept map Concept Knowledge
chapter 1 Developing Yourself as an Effective Learning and Development Practitioner In t r od u c t Io n This chapter begins with a look at what is required of L&D professionals and how L&D roles are specified. We discuss the CIPD HR Profession Map and how we can use it to assess our professional development needs. We then move on to look at how we deliver our L&D service‚ considering: who are our customers‚ how well do we meet their needs and what can we do to improve our service delivery. In
Premium Personal development Copyright Human resource management
Cited: Johnson‚ Steven. The Ghost Map. New York City: Penguin Group USA Inc. ‚ 2006.
Premium Epidemiology Water supply Cholera
Molecule Polarity PhET Lab A study of electronegativity‚ bond polarity‚ and molecular polarity Introduction: In this atomic-level simulation‚ you will investigate how atoms’ electronegativity value affects the bonds they produce. When two atoms bond‚ a pair of electrons is shared between atoms. Electronegativity is a measure of a single atom’s ability to hoard electrons shared in that bond. In this lab you will work diligently‚ at your own pace‚ to answer a number of questions. To begin‚ from
Premium Atom Chemical polarity Chemical bond