of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the reaction on the pectinase
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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Chemical reactions are a part of everyday lives. Whether it is the burning of propane at a kitchen stove‚ or washing detergent reacting with dirt stains‚ chemical reactions are useful tools that have widespread applications. With so many reactions assisting our everyday activities‚ it is important that ways to optimise reaction rates are investigated. “Reaction rate‚ the speed at which a chemical reaction proceeds… may be defined in terms of the amounts of the reactants consumed or products formed
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98.) Consider the unbalanced redox reaction: Cr2O72- (aq ) + Cu(s ) → Cr3+ (aq ) + Cu2+ Balance the equation in acidic solution and determine how much of a 0.850 M K2Cr2O7 solution is required to completely dissolve 5.25 g of Cu. Nitric acid is usually purchased in concentrated form with a 70.3% HNO3 concentration by mass and a density of 1.41 g/mL. How much of the concentrated stock solution in milliliters should you use to make 2.5 L of 0.500 M HNO3? Mass %= Mass Solute/ Mass Solvent + Mass
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Introduction‚ aim and hypothesis Chemical reactions are accompanied by the release or absorption of energy. Reactions which release energy are described as exothermic and those which absorb energy are endothermic. The energy released in chemical reactions was previously stored as chemical potential energy in the reactants; this stored energy is called enthalpy. The aim of this practical is to observe and classify chemical processes as endothermic or exothermic‚ based on the changes in temperature
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Rate of Reaction Introduction Hydrogen Peroxide (H2O2)‚ is the most simple peroxide and commonly used in several household items such as toothpaste or as an alternative to bleach. However hydrogen peroxide it is a very dangerous substance when accumulated in large amounts. If that situation occurs this substance must be decomposed. But how can we decompose this reaction? Well it decomposes by itself very slowly (2H2O2(aq) -> 2H2O(l) + O2(g))‚ but that would take too long. This reaction can be sped
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Experiment three was divided into three parts; the preparation of the solutions‚ the reaction between Hydrochloric Acid and Ammonium Hydroxide (part b)‚ and the formation of Magnesium Oxide (part c). 200 mL of deionized water were added to a beaker followed by the addition of 100 mL 6 M HCl‚ which reacted to make 300 mL of a 2 M HCl solution used for Part B. Next‚ 50 mL of deionized water were added to a separate beaker and then 100 mL 3 M NaOH were added to the beaker to form 150 mL of a 2 M NaOH
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The SN2 reaction requires three qualities: a strong nucleophile‚ a good‚ unhindered leaving group‚ and a polar‚ aprotic solvent. For our reaction‚ we have all three bases covered. The nucleophile is an alkoxide‚ a deprotonated alcohol. Technically‚ because our alcohol is a phenol‚ the conjugate base is called a phenoxide. Phenol itself has a pKa of about 10‚ but our alcohol has more resonance opportunities‚ so the pKa is down around 8. This is sufficiently acidic for use of weak base like K2CO3 for
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All chemical reactions are processed through energy exchanges. Chemical reactions either absorb energy or release energy into their surroundings. In thermodynamics‚ endothermic reactions absorb energy where exothermic reactions release energy. In this experiment‚ we will be observing the chemical reactions that occur when a specific liquid is combined with a specific solid. We will measure the temperature of the liquid before the solid is added. Then we will measure the temperature of the liquid
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Diels-Alder reaction was performed in this experiment. Which is a reaction that was discovered by Otto Diels and Kurt Alder. This particular type of reaction is the concerted cycloaddition among a dienophile and a diene. The Diels-Alder reaction allows for the synthesis of stereospecific rings in an efficient manner. This reaction proceed in a single step method‚ otherwise known as Nucleophilic Substitution in the second order (SN2). The Diels-Alder reaction is categorized as a pericyclic reaction‚ this
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