The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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It was originally hypothesised that the reaction time would decrease with higher concentrations of the reactants‚ potassium iodate and sodium bisulfite as collision theory stated that more molecules present in a given volume meant a greater chance they would collide. Furthermore‚ the time was expected to decrease with higher temperatures as it influenced the reactant particles by raising their kinetic energy. However‚ the data collected from this experiment only partially supported this hypothesis
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Constant of an Unknown Ester Hydrolysis Reaction Abstract The experiments to follow determined that the equilibrium concentrations of the reaction: ester + water ↔ alcohol + acid‚ are equal to 0.0363 moles of ester‚ 0.2852 moles of water‚ and 0.0268 moles each of alcohol and acid. Using this information the equilibrium constant was determined to be 0.06938. 1. Introduction In this lab the equilibrium constant‚ Kc‚ for the acid catalyzed reaction between an unknown ester and water
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Abstract The photocatalytic degradation of phenol has been tested primarily with titanium dioxide (TiO2) either alone or in combination with other materials. In this work‚ Mg/Al hydrotalcites prepared by two methods using inorganic (HC) or organic (HS) chemical reagents‚ along with mixed oxides produced by calcination of these products (HCC and HSC) were mixed with titanium isopropoxide to obtain hydroxide-TiO2 compounds (HCC-TiO2 and HSC-TiO2) to test their photocatalytic activity compared with
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experiment the objective was to perform a Diels-Alder reaction using cyclopentadiene and maleic anhydride to synthesize the product‚ cis-Norbornene-5‚6-endo-dicarboxylic anhydride. The Diels-Alder reaction is one of the most important reactions in organic chemistry and was first investigated by Otto Diels and Kurt Alder in Germany. It is a [4+2] concerted cycloaddition reaction which involves a diene and a dienophile. The Diel-Alder reaction are mainly used for creating new carbon-carbon bonds‚ mostly
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Soda and Mentos Reaction: Any kind of Soda and Mentos interaction will expedite a geyser of both carbon dioxide and foam. Soda has a few properties‚ for instance‚ an sweetener or an artificial flavouring‚ but it’s main factor that contributes to the reaction is carbonated water. Carbonated water is basically water with Carbon Dioxide compressed within it‚ it causes the drink to be fizzy and bubbly. This contributes to the reaction because it the fizz and bubbles are gas‚ which tries to escape from
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Discussion The purpose of this lab is to display the influence of retronasal olfaction when it comes to flavor. Humans have two different types of olfaction. Olfaction is the detection of chemicals in the air. These chemicals which are meant to activate odor receptors‚ are called odorants. Humans have approximately 20 million olfactory receptors‚ which is only a fraction compared to bloodhounds who have 4 billion. The first type of olfaction is called orthonasal olfaction which is inhaling. Here
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1. Determine the order of the reaction A --> 2B + C from the following data obtained for [A] as a function of time. time | [A] | 0 min | 0.80 M | 8 mins | 0.60 M | 24 mins | 0.35 M | 40 mins | 0.20 M | ------------------------------------------------- Use diferential and half life. 2. Balance the equation below and determine the rate expression using the given data. Find k with units. N2 + H2 ---> NH3 (all gaseous) [N2] (mole/L) | [H2] (mole/L) | Initial Rate (mole/L /min)
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test tube D and E. The metal spreader was then sterilized again for any future uses. These plates for C‚ D‚ E‚ and the streaking plate were left upside down‚ taped and labeled with the section and lab ID number and then incubated for 2 days at 30 degrees Celsius and refrigerated until the following lab
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The purpose of this review paper is to discuss the effect of temperature on enzyme- catalyzed reactions. This is relevant because many diseases can be diagnosed and controlled by the processes of enzyme activity (Worthington 2015). If more information is not found about enzyme activity and how it is affected‚ many diseases may go undiagnosed and uncontrolled. Temperature is a type measurement that does not only consist of heat. It is the measurement of the mean kinetic energy of any group of particles
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