University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
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The progress of the reaction was monitored in my case using two TLC plate. It first started off with the spotting of Standard benzoin and benzil which were provided in the lab and followed by the addition of the reaction mixture at once it starts changing colour/boiling‚ then at 10 and 20 mins into the reflux. Once all the necessary steps were spotted‚ the TLC plate was placed in in a beaker containing CH₂Cl₂(methylene chloride)‚ which was used as the developing solvent in this experiment. To check
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details Results and Discussion: Voltaic Cell Reactions | | | Overall Cell Reaction | Observed Voltage | Theoretical Voltage | Cu2+(aq)+Zn(s) Cu(s)+Zn2+(aq) | 0.947 V | 1.10 V | Cu2+(aq)+Sn(s)Cu(s)+Sn2+(aq) | 0.571 V | 0.473 V | Cu2+(aq)+Fe(s) Cu(s)+Fe2+(aq) | 0.512 V | 0.777 V | Cu2+(aq)+Mg(s) Cu(s)+Mg2+(aq) | 1.598 V | 2.707 V | Cu2+(aq)+Pb(s) Cu(s)+Pb2+(aq) | 0.651 V | 0.463 V | Concentration Cell | Based on Cu2+ - Cu | Cell Reaction | | [Cu2+] anode | [Cu2+] cathode | Observed
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Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water
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Period : 4 Grade : 11 Enzyme Lab Report Question How heats effect the length of reaction time of an enzyme? Hypothesis I think the heat will make the length of reaction time of an enzyme become slowly. Heat is one of a way to denature the substrate. It means the heat will break down the structure of substrate in order the reaction of enzymes that we activated into it become slowly. Method for Collecting Data First I will record the length of reaction time with stopwatch when I pour
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A double displacement reaction is a type of chemical reaction in which two compounds react in order to create two new chemicals. The two cations and anions switch places with each other which forms two new compounds. As an example AB+CC can switch to AD+CB. Both sides of these equations must balance out in order to be neutral. So any pairs of compounds (such as A+B) must have a total balanced charge. In this example A could have a charge of positive 2 (+2) and B could have a charge of negative
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Hugh Kim Lab Report: Stoichiometry Lab 1. Prelab Part1. 1) Create no waste = The principle that encourages chemists to not create waste at the first place rather than cleaning it up afterwards effectively shifts the chemistry more environmentally conscious‚ as creating no waste would make the experiment efficient; the reactants will be reduced to only the essential ones and the product will be maximized‚ a change that would make the experiment economic. Also‚ if chemists aim to
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Enzyme Lab Report Introduction: Enzymes are proteins that enable chemical reactions. In the enzyme lab‚ the effects of concentration‚ temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction. Materials and Methods: Experiment 1‚ 2‚ & 3 Experiment 1 examined the effects of concentration on catalase activity. Experiment 2 examined the effects of concentration in temperature
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everyday lives. Many artists today even try to mimic artists in ancient Greece‚ but often fail to do so. One of the most important aspects to come out of Greek art is the architecture‚ most notable the three architectural orders of ancient Greece‚ Doric‚ Ionic and Corinthian. Each components of this order has a system of inter dependable parts whose proportions are based upon mathematical ratios. The basic components of all three orders are the column and entablature. Although most people do not know this
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