Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
Premium DNA Molecular biology Bacteria
on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials
Premium Staphylococcus Bacteria Staphylococcus aureus
Preparation of t-Butyl-Chloride March 8 & 15‚ 2012 Theory: Alkyl halides can be synthesized when alcohols react with hydrogen halides. An alkyl halide is a halogen-substituted alkane‚ and a hydrogen halide is a compound consisting of a hydrogen bonded to a halogen (H-X). Alkyl halides are classified as primary‚ secondary‚ or tertiary depending on the number of alkyl substituents directly attached to the carbon bearing the halogen atom. The purpose of this laboratory experiment was to prepare
Premium Sodium chloride Hydrochloric acid Chlorine
The poem “ The Unknown Citizen By W H Auden is a poem that pokes fun at the life of a automaton working in a factory. Reading it in a glance‚ I find the title that catches my very first attention. It is because Auden gives the poem such a title that the whole poem sounds so dramatic and pompous‚ as if it is dedicated to some citizen who have sacrificed so much for the nation. The citizen‚ to my dismay‚ is never named and remains as the unknown one throughout the whole poem. The citizen is referred
Premium W. H. Auden Government The Unknown Citizen
Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
Premium Bacteria Microbiology Escherichia coli
Determination of KHP – Acid - Base Titration For determining purity of unknown KHP‚ acid – base titration was used. Molarity of titrant in this case NaOH was standardized as 0.04958(±0.00011) M. Purity of unknown KHP was calculated as 35.88(±0.16) %. • Introduction Acid – base titration is typical method of determining unknown figures. Acid – base titration means that by using neutralization between acid and base. Abrupt changing of pH says whether procedure is done or not. pH indicators
Free Titration PH indicator PH
Tiffany Ward Scheme For Unknown 2A/B Possible Ions: Cations: Na+‚ K+‚ NH4+ Anions: OH-‚ Cl-‚ NO3-‚ SO42-‚ HSO4- 1.Describe Sample Phase‚ color‚ odor‚ crystal shape‚ amorphous‚ gel-like‚ needle-like‚ powdery‚ etc. 2.Tests on original sample (i) Flame test bright orange Na+ purple K+ no color NH4+ (ii) NH4+ test Place a small portion of original unknown sample in a test tube or spot plate‚ then add a few drops of 1M NaOH solution. At the same time suspend a piece of red
Premium Solubility Potassium Solid
"The Unknown Citizen" is an occasional poem. That is to say‚ it is a poem written to mark a specific occasion or event. The occasion is indicated in the lines contained in parenthesis that precede the body of the poem. As these lines indicate‚ the poem is a written monument that functions like a cenotaph: it commemorates a fallen man whose identity is unknown. However‚ unlike the soldier who falls in a battle of war‚ the battle this individual appears to be unwittingly a part of is a social battle
Premium Rhyme Grammar Poetry
INVESTIGATION #2 DETERMINATION OF THE WATER OF CRYSTALLISATION April 4‚ 2012 OBJECTIVE: Chemical compounds that contain discrete water molecules as part of their crystalline structure are called hydrates. Hydrates occur quite commonly among chemical substances‚ especially among ionic substances. More often than not‚ such compounds are either prepared in‚ or are recrystallized from‚ aqueous solutions. Hydrates exist for ionic compounds most commonly‚ but hydrates of polar and non polar covalent molecules
Premium Water Chemistry Oxygen
Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the
Premium Bacteria Gram staining Staining