the mixture of individual compounds from the spinach leaf sample used. Material and apparatus: Part 1 : Analysis of Analgesic Drugs Material: Aspirin Acetaminophen Caffeine Unknown A Unknown B TLC plates Ethyl acetate Hexane Acetic acid Iodine Apparatus: UV Lamp Capillary tube 250mL beaker Filter paper
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and nitrate(NO2-). The group reagent is dilute hydrochloric acid. EXPERIMENT Salt + dil. HCl OBSERVATION Effervescence or evolution of gases (a) Colourless‚ odourless gas turns lime water milky (b) Colourless with rotten eggs smell and turns lead acetate paper black. (c) Colourless gas with suffocating smell. Heat and pass the gas through acidified K2Cr2O7 solution. The solution turns green (d) Colourless gas with suffocating smell with a yellow ppt. of sulphur in the test tube. Heat and pass the
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Mapua Institute of Technology Organic Chemistry Laboratory 2 Final Report Factors Affecting the Relative Rates of Electrophilic Aromatic Substitution Reaction Justiniano‚ Priscilla Raiza N. School of Chemical Engineering and Chemistry‚ Mapua Institute of Technology‚ Intramuros‚ Manila‚ Philippines Experiment No.1‚ Submitted on August 6‚ 2011 at N402. Abstract EXPERIMENT NUMBER ONE IS ALL ABOUT THE ELECTROPHILIC SUBSTITUTION OF AROMATIC COMPOUNDS. AROMATIC COMPOUNDS ARE THOSE ORGANIC
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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Comparative Investigation of Organic Compounds Tristan Duane G. Lacson‚ Lara Elize T. Lamigo‚ Maria Mikaela L. Laysa‚ Christian Gerard G. Lee and Karen L. Li Group 6 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In order to differentiate the organic compounds. The group tested the compounds using the information based on their instinsic physical properties‚ behavior in ignition‚acidity and basicity and their infrared spectra. The physical properties were noted by the reaserchers by
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concentration i.e. 50- 500 μg/ml‚( each of these concentration was taken in duplicate) was mixed with 1800 μl of ABTS solution was mixed with it for each concentration. Similar process was repeated for ascorbic acid concentration. All test tubes (ethyl acetate soluble fraction‚ ethyl-3-hydroxy-5-methoxy-4-methylbenzoate‚ ascorbic acid‚) labeled separately were shaken well and incubated in the dark at 25oC for 30 minutes. Then the Kinetic absorbance was recorded at 734 nm by spectrophotometer after 1 and
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The Role of the Citric Acid Cycle The Citric Acid Cycle is a series of enzyme-catalysed reactions that take place in the mitochondrial matrix of all aerobic organisms. It involves the oxidation of the acetyl group of acetyl CoA to two molecules of carbon dioxide. Each cycle produces one molecule of ATP by substrate-level phosphorylation‚ and reduces three molecules of NAD and one molecule of FAD for use in Oxidative Phosphorylation. The cycle is preceded by Glycolysis‚ which also occurs in anaerobic
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pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on differences in their physical properties. It can be used as a method of purification (and can be used on a
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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Determination of Caffeine in Tea Samples ACKNOWLEDGEMENTS First of all T would like to thank my cousin brother Mr. Neeraj Naithani Research Scholar Department of Chemistry‚ TTT Roorkee for his contribution in this study. T would also like to thank my Chemistry Teacher Ms. Rampal in providing a helping hand in making my project titled “Determination Of Caffeine In Tea Samples”. Disclaimers: The errors idiocies and inconsistencies remain my own. Ankit Bahuguna (Name and signature of the student) Determination
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