1. The overall goal of this lab was to carry out experiments that clearly demonstrate the different ways DNA genetic information‚ specifically transduction and conjugation. The first half of this experiment focused on exploring the mechanisms of transduction. This was done by creating a spot titer plate for phage carrying kanamycin resistance and E. coli. E. coli was then proven to have gained kanamycin resistance throughout transduction as demonstrated by its ability to grow on a medium containing
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determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite. In the second part of the experiment‚ the effect of pH was examined. Using the buffered solutions: acetate solutions (pH 4 and 5)‚ phosphate buffer (pH 6.7 and 8)‚ and bicarbonate buffer (pH 10). The results were recorded having all of them as infinite. Keywords: enzyme‚ infinite‚ pH‚ temperature‚ salivary amylase‚ starch I. Introduction Enzymes
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Department of Chemistry University of Kentucky EXPERIMENT 5 Molecular Absorption Spectroscopy: Determination of Iron With 1‚10-Phenanthroline UNKNOWN Submit a clean‚ labeled 100-mL volumetric flask to the instructor so that your unknown iron solution can be issued. Your name‚ section number‚ and locker number should be written legibly on this flask. The flask does not need to be dry on the inside‚ but needs to have been rinsed with distilled water after it has been washed. The flask
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Chemistry 1: PROBLEM SET SY 2012/2013 CLASS #: _______________ NAME_________________________________________ SECTION: ______________________ Stoichiometry II - Mole Calculations/ Limiting and Excess Reagent – Lecture Notes 1. Given the balanced equation N2(g) + 3H2(g) 2NH3(g) How many moles of ammonia are produced when 0.60 mol of nitrogen reacts with hydrogen? 2. Given the equation: SiO2 + HF SiF4 + H2O a. Calculate the number of moles HF that would completely react with 2.5 moles of
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Aloe (Aloe vera) is a major and traditional medicinal plant under family of Liliaceae. Aloe consumed externally and internally as well. It is used externally to treat several skin burn conditions‚ sunburn and poison oak‚ infections‚ and eczema. It is confirm that juice from Aloe vera ease pain and decrease inflammation. It has antiseptic and antibiotic properties in treating cuts and abrasions. Aloe juice used to treat people with ulcerative colitis‚ an inflammatory bowel disease. Moreover it acts
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Enzymes and their importance in plants and animals (25 marks) Enzymes are biological catalysts‚ which accelerate the speed of chemical reactions in the body without being used up or changed in the process. Animals and plants contain enzymes which help break down fats‚ carbohydrates and proteins into smaller molecules the cells can use to get energy and carry out the processes that allow the plant or animal to survive. Without enzymes‚ most physiological processes would not take place. Hundreds of
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Variety Variety is the spice of the Barbie Doll Marketing Success. First introduced to the US market in 1959‚ Barbie has grown into a global cultural phenomena. From just one female doll‚ the Barbie family has grown and includes over 38 friends and relatives. There are now dolls that represent multiple ethnicities‚ males‚ different age groups‚ different abilities‚ different lifestyles‚ etc. All totaled‚ there are now over one hundred and fifty different dolls under the Barbie brand. Quality
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negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH nullifies the negative charge on the protein
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pH MEASUREMENT AND BUFFER PREPARATION ! ! 2A—Pharmacy‚ Faculty of Pharmacy‚ University of Santo Tomas Canare‚ Ann Shery; Caraos‚ Aeraille Diane; Carillo‚ Pauline Mari; Carreon‚ Leslie Ann; Co‚ Alexandra Kay !! !! ABSTRACT. The regulation of internal pH is a physiological function of major importance for all organisms. The quantity pH is intended to be a measure of the activity of hydrogen ions in solution. Buffers‚ on the other hand‚ are solutions containing a substantial concentration of both
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Chemistry of Spices This page intentionally left blank Chemistry of Spices Edited by Villupanoor A. Parthasarathy Indian Institute of Spices Research Calicut‚ Kerala‚ India Bhageerathy Chempakam Indian Institute of Spices Research Calicut‚ Kerala‚ India and T. John Zachariah Indian Institute of Spices Research Calicut‚ Kerala‚ India CABI is a trading name of CAB International CABI Head Office Nosworthy Way Wallingford Oxfordshire OX10 8DE UK Tel: +44 (0)1491 832111 Fax:
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