coagulation floated to the surface of solution. The curve of succinylated egg white did not change much. The –NH3+ of protein in egg white was replaced by COO- at pH8‚ so the protein was negative charged. The fastest coagulation of protein is at pI (isoelectric point). Because the pH of solution was at pI‚ the whole process is slow. The aim of this period of experiment is calculating the percentage of succinylation of the sample. Ninhydrin reacts with –NH2 to give a purple colour. There are three samples
Premium Protein Egg white Isoelectric point
1) Comment on the pros and cons of using CM-5 chips in SPR analysis (3 marks). CM-5 chips can provide a relatively high carboxylation of dextran matrix which develops high negative-charged surface that contribute to bind large amount of ligands. However‚ the high negative-charged surface may increase the chance of non-specific binding of other stronger positively charged molecules causing false positive signals. Covalent bound with the ligand through the EDC/NHS activation process
Premium Protein Blood Isoelectric point
into the first main point of the body. A connective is not required here but may be used if desired. For information on connectives‚ review pages 177-178 of your textbook. Skip a space above and below connectives.) BODY I. A single complete sentence expressing the main point of this section of the speech A. Sub point [As with main points‚ sub points should be written in full sentences.] 1. Sub-sub point [Write sub-sub points in full sentences.] 2. Sub-sub point B. Sub point moving from the introduction
Premium Linguistics Sentence Logic
“cut-throat” competition. Automation of one’s point of sale and inventory system puts you a step ahead in the game. It helps you conduct business transactions with ease as you only have to press a few keys. It liberates you from countless paper works as computation of the day’s sale and keeping track of inventory are done automatically. With these things in mind‚ the proponents hopes that upon completion of this Proposed Point of Sale and Inventory System‚ will find it of great
Premium Receipt Computer The Sale
Body: Each of your supporting points in your defense will need a paragraph. State your point as a way of creating a topic sentence for an evidence paragraph. You’ll need at least three and probably no more than five. Each evidence paragraph should have specific support by way of facts‚ statistics‚ examples‚ or other details. You may use personal example as some of your supporting detail‚ but try to use more objective support as well. Body: Each of your supporting points in your defense will need a
Premium Point Support Technical support
Title Freezing and Melting Point Objectives 1. To gain proficiency in constructing a graph and plotting data points. 2. To determine the freezing points of a compound from the graph of decreasing temperature versus time. 3. To determine the melting points of a known and unknown compound. Introduction Physical changes are the changes in the physical properties of a substance (Moore‚ Stanitski & Jurs‚ 2009). Freezing point is the fixed temperature at which a pure liquid converted into crystalline
Premium Melting point Liquid Chemistry
in life is nice and easy.” Mia lost her parents and brother. Mia has to make a decision between life and death. Mia got into a big car accident that put her in a coma. Point of View This book is told in the point of view of first person. Mia tells the story through her own eyes. The author also says “I” and “we”. The point of view of this story helps the reader understand how Mia feels and shows us what she sees through her own eyes. If the author were to chose third-person
Premium Book Death Automobile
Reliance Case Questions (12 Points Possible) A. Situation Overview (2 Points Possible) What are the strengths and weaknesses of the RBS brand? You clearly identified issues related to the central decisions in the case. Your analysis was clearly linked to facts from the case. (2 Points Earned) Your analysis was unclear or was not supported by facts from the case. (1 Point Earned) Substantive omissions were made in your analysis. (0 Points Earned) B. Past Promotional Events (2 Points Possible) Analyze the
Premium Strategic management Marketing Strategy
Engineering and Chemistry Muralla St.‚ Intramuros‚ Manila Melting Point and Boiling Point of Organic Compounds Group No. 5 Manacup‚ Cris Vincent L. Oblena‚ Adrian D. Ong‚ Joshua Jyro B.* *Leader ABSTRACT In compounds‚ two of the physical properties affected by the varying structures are melting point and boiling point. Through the use of the Thomas-Hoover Melting Point Apparatus or the micro method‚ the melting and boiling point of organic compounds are now determined. The aim of this experiment
Premium Hydrogen bond Carboxylic acid Melting point
Melting Point Determination Identity and Purity of Solid Organic Compounds Objectives • To introduce the technique of melting point determination. • To use the concept of melting points for identification and characterization of organic compounds. • Properly fill and use a capillary melting point tube. • Determine accurate melting point ranges for a wide variety of organic substances. Introduction The melting point of a solid can easily and accurately be determined
Premium Temperature Melting point