Mali Lewis Communication 1310 Cattle Identification in Commercial Agriculture Topic: Methods of Cattle Identification Organization: Topically Specific Purpose: I want my audience to understand the different methods of cattle identification that are used in the commercial agriculture industry. I. Introduction A. Attention getter: A traditional West Texas saying goes‚ “trust your neighbors‚ but brand your cattle.” B. Relevance: Commercial agriculture contributes to the food we eat‚ and through
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Isolation and Study of Organelles from Pea Seeds by Differential Centrifugation and Corn to Determine Mitochondrial Activity BIOL 230-01 Cell & Molecular Biology October 13‚ 2014 ABSTRACT Since the discovery of the cell in 1665 by Robert Hooke‚ man has always wondered about the complexity and potential functionality. Using pea seeds‚ which is readily available‚ we will be studying the different parts of the cell by separating it into its component parts by
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E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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Running head: DNA Evidence and Job Description/Ethics Name Course Tutor Date How would the environment affect this fragile evidence? II. Collecting Fingerprints from a Weapon Describe how you would collect a fingerprint from a weapon that could possibly have touch DNA on it as well. UNIT 9: Job Description for Latent Print Examiner Write a job description for a Latent Print Examiner. Latent Print Examiner Salary scale: Between $ 70‚000 and $
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DNA FINGERPRINTING DNA fingerprinting is a method of identification that compares fragments of deoxyribonucleic acid. It is a technique used to distinguish between individuals of the same species by using only samples of their DNA. It is also called DNA typing. DNA is the genetic material found within the cell nuclei of all living things. In mammals‚ the strands of DNA are grouped into structures called chromosomes. Unless dealing with identical twins‚ the complete DNA of each individual is unique
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and the discovery of DNA Ralf Dahm* Max Planck Institute for Developmental Biology‚ Department 3 – Genetics‚ Spemannstr. 35/III‚ D-72076 Tubingen‚ Germany ¨ Received for publication 5 October 2004‚ revised 17 November 2004‚ accepted 20 November 2004 Available online 21 December 2004 Abstract Over the past 60 years‚ DNA has risen from being an obscure molecule with presumed accessory or structural functions inside the nucleus to the icon of modern bioscience. The story of DNA often seems to begin
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An Introduction to DNA microarrays Rebecca Fry‚ Ph.D. http://www.buffalo.edu/UBT/UBT What is a DNA Microarray? genes or gene fragments attached to a substrate (glass) Tens of thousands of spots Hybridized slide Two dyes Image analyzed 1 The Beginnings of Microarray Technology Lockhart et al.‚ 1996 Nature Biotechnology “Expression monitoring by hybridisation to high-density oligonucleotide arrays” Schena et al.‚ 1995 Science “Quantitative monitoring of gene expression
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Experiment #9 Isolation and Isomerization of Lycopene from Tomato Paste Goal / Purpose To extract the carotenoid pigments from canned tomato paste and separate them by column chromatography to obtain a solution that contains lycopene. Then record the ultraviolet-visible spectrum of this solution and analyze it for evidence of isomerization. Calculation List of Materials 4.0g tomato paste screw cap vial Chromatography column 25ml buret 50ml flask ring stamd and clamp 50% mixture
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Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase support
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Chirality – Isolation of Limonene from Citrus Fruits Introduction: Terpenes are a large and varied class of hydrocarbons produced by a wide variety of plants. Many essential oils belong to the terpene class. They are made up of two or more five carbon units that are called isoprenes. Limonene is a terpene that can be separated from orange peels almost 100% in the (R)-(+)-limonene form. Other citrus fruits like lemons‚ grapefruits‚ limes‚ and tangerines contain the compound as well. The
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