ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
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Isolation Have you ever felt isolated from your surroundings‚ either because of language or culture wise‚ being new in town‚ feeling friendless‚ or simply because you have to study while everyone else is out having fun while you study? Well I have had many experiences like those. There was a time in which I had moved from New Jersey to Tennessee and I was the new kid on the block. Also there was another addition in my life
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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Isolation and identification of pathogenic bacteria associated with frozen mackerel fish (Scomber scombrus) in a humid tropical environment Eze‚ E. I. 1 ‚ Echezona‚ B. C. 1 * and Uzodinma‚ E. C. 2 1 Department of Crop Science‚ University of Nigeria‚ Nsukka‚ Nigeria. 2 Department of Science Laboratory Technology‚ University of Nigeria‚ Nsukka‚ Nigeria. Accepted 8 January‚ 2010 Aquaculture products can harbour pathogenic bacteria which are part of the natural micro-flora of the environment. An in-vitro
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The poem‚ “Monologue for an Onion” written by Sue Kim‚ the onion is a metaphor and is the whole poem is written about it and based off of it. The basic main viewpoint of the poem is about the feelings and thoughts that onion experiences when being handle by the person or more specifically when it is being cut. The poem revolves around the whole idea of how the onion feels and thinks as the person cuts away at it and how it reflects on the actions of the person and the person themselves. Sue Kim’s
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MONOLOGUE FOR AN ONION: AN ANALYSIS Cutting an onion breaks cells in its skin causing a reaction that results to the formation of volatile sulfur compound which‚ when mixed with tears coating our eyes‚ becomes sulfuric acid. This triggers the tear ducts to produce more tears to alleviate the imbalance in acidity the irritant has caused; the longer the eyes are exposed to the acid the greater the quantity of tears produced. Sue Kwock Kim uses this certain property of an onion to tackle a specific
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Extraction of Caffeine from Lipton® Tea Leaves Nathaniel Camangon*‚ Kaila Bumagat‚ Franz Mari Burgos‚ Remigio Callanta Department of Chemistry‚ College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment mainly focuses on the extraction of pure caffeine by multiple extraction from commercially acquired tea leaves and determining its purity by melting point determination. A 0.02% yield of pure caffeine was obtained from 10.4978g of tea leaves (4 tea bags).
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The Onion Cell Lab Background: Onion tissue provides excellent cells to study under the microscope. The main cell structures are easy to see when viewed with the microscope at medium power. For example‚ you will observe a large circular nucleus in each cell‚ which contains the genetic material for the cell. In each nucleus‚ are round bodies called nucleoli. The nucleolus is an organelle‚ which synthesizes small bodies called ribosomes. Ribosomes are so small you cannot see them with the light microscope
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Extracting DNA from Human Cheek Cells: Conclusion The hypothesis of my experiment‚ if the strawberry DNA product was strandlike yet clumply then human epithelial cell DNA will be strandlike and clumpy too‚ was supported. This is because through the observations noted when the lab was being conducted‚ it can be seen that the human epithelical cell DNA was small‚ stringy‚ clumpy and squishy‚ just like the strawberry DNA product. Other observations of the human epithelial cell DNA was it was not only
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Satirical Essay With an increase in consumer culture and a minimal amount of common sense‚ the onion uses a satirical tone to draw attention to the consumer’s gullibility and the power of the advertising industry. Through subtle humor‚ the Onion is able to successfully mock the intriguing diction of genuine advertisements. The advertisement claims that through “no fewer than five forms of pseudoscience”‚ Magnasoles are able to “stimulate and soothe” consumers feet. This directly mocks the unanimous
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