Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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Isolation has a poor connotation attached to it because numerous people fear being alone. Society reinforces that notion by declaring that to be happy we need to be married‚ have kids‚ own a house and be social. Andrew Wyeth’s painting Wind from the Sea fights this common belief by portraying the beauty and simplicity of a remote location. In the painting‚ the curtain is being blown by the wind and beyond the window the only commodity you can see is the sky‚ the bit of snow that has not yet melted
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Practical guide to detection and identification of Phytophthora Leaf blight A number of Phytophthora species cause leaf blight. These include: P. infestans on potato and tomato; P. palmivora on a large number of tropical fruit species including rubber‚ durian and macadamia; and P. colocasiae on taro. These blights on leaves are first seen as small flecks but within 3-5 days they expand to produce large lesions. Initially‚ infected tissue is water soaked but becomes necrotic (brown or black) in
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and
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any specific mineral. Well‚ “because the atomic structure of a mineral species is always the same‚ most of its physical properties are relatively constant and may be used for the mineral’s identification” (Gardiner and Wilcox 107). There are many intriguing physical properties to mineral identification. Visible properties include color‚ luster‚ streak‚ crystal form‚ cleavage. The tactile property can be measured using Mohs scale‚ which measures hardness of minerals compared to common objects
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resultant solution diluted up to the mark with diluents to obtain 1000 μg/ml. Further stock solution was used to prepare plasma standards to construct the calibration curve. C. Pre-chromatographic isolation of baclofen from plasma Prior to chromatography‚ baclofen from plasma constituents was isolation by adding 0.25 ml of acetonitrile and approximately 25 mg of zinc sulphate crystals to 0.50 ml of plasma. The aged plasma was stored at - 250C. This plasma was thawed at room temperature and 0.50 ml
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grow onions Today‚ most people simply eat onions raw or cooked‚ in about a thousand different ways. Onions are warm-season veggies and‚ however you plan to use them‚ you can grow them anywhere following the following easy steps: a) Buy started plants at the nursery. Otherwise‚ start seeds indoors 8 to 10 weeks before the last expected frost (see "How to Start Vegetable Seeds Indoors"). b) Choose a site that gets at least 6 hours of sun a day and has soil with a pH of 6.0 to 7.5. Onions need moderately
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the public’s gullibility is optimized by the marketing industry’s power. The Onion‚ a publication devoted to humor and satire‚ derides how products are marketed to consumers in order to illustrate the absurdity of marketing strategies. The Onion utilizes testimonials and clever diction to satirize the methods availed by marketers. The Onion presents personal testimonials to mock how marketers employ false claims from allegedly real customers to sell their product. Helene Kuhn mentions she twisted
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Table of Contents Individualism and Collectivism in China 1 What is Individualism and collectivism? 1 Ritual: 2 Hero: 2 Symbol 3 Individualism vs. Collectivism Onion Model 4 Human Nature: 4 Country: 4 Case: 5 Individualism and Collectivism in China What is Individualism and collectivism? As we learnt in CCA‚ collectivism is natural and means that people will stay loyal to their in-group in return for acceptance and protection. Most societies are collectivistic with a few
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Identification of Metallic Ions Peter Jeschofnig‚ Ph.D. Version 42-0160-00-01 Kellie Long Purpose: To observe the different flames of alkali and alkaline metals. Procedure: Place a little bit of each element into the well plate. Then you soak a tip of a cotton swab in each solution. Then you place the cotton swab over the Bunsen burner until the flame changes color. observations The color of the flame is expected to be as follows: Metallic ion Flame color
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