Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology Dissecting set
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------------------------------------------------- Isolation of Caffeine from Coffee Date of laboratory work: 10/9/2012 5:00-7:45 Date of report written: 10/15/2012 Introduction: The purpose is to isolate caffeine from coffee by using the organic solvent dichloromethane (CH2Cl2) that is also referred to as direct contact. Calcium carbonate (CaCO3) is used to remove the tannins from the coffee. Afterwards an extraction is used to separate the organic layer (bottom) from the aqueous layer (upper). By
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Name: Date of Experiment: November 20‚ 2012 Group mates: Date of Submission: November 28‚ 2012 Experiment No. 1 ISOLATION OF CAFFEINE FROM TEA I. Introduction Caffeine is a very common substance and is found in coffee‚ tea‚ soft drinks‚ chocolate‚ and "stay-awake" pills such as Vivarin. It can be synthesized or isolated from natural sources. Caffeine constitutes approximately 4% of tea and coffee leaves‚ and is also found in cola nuts and cacao beans. Caffeine is a member
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BACTERIA Period: 4 Characteristics: 3 major shapes Cocci Basilli Spirilla 3 major components Mesosomes flagella Plasmids Growing Up: Bacteria can obtain energy through phototrophs(sunlight)‚ lithotrophs(inorganic compounds)‚ and organotrophs(organic compounds) Marriage/Reproduction Binary Fission: The process by which all bacteria reproduce. It results in the separation of a single cell into two. Transformation: genetic alteration
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“Fighting Invisible Killers” Scholastic Scope: The Language Arts Magazine January 2014 Edition‚ pages 5-9 Bacteria surround us every day. These little “bugs” are invisible to the eye and most do not harm us. Many are necessary for us to survive‚ like the bacteria in our stomachs and intestines that help us to digest food. But some bacteria are very dangerous to us. Addie Rereich became very sick in May 2011‚ when she was 11 years old. What started as something that her doctors and mother
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Isolation of Casein from Milk In this experiment‚ casein was isolated from milk by means of isoelectric precipitation. A percent yield of % was obtained by the group. Introduction Milk is an opaque white or bluish-white liquid secreted by the mammary glands of female mammals‚ serving for the nourishment of their young. This liquid‚ as secreted by cows‚ goats or certain other animals are used by humans as food and as a source of dairy products such as cheese and butter. Milk composition differs
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Isolation of Trimyristin from Nutmeg by Extraction Introduction: Extraction is the physical process by which a compound or mixture of compounds is transferred from one phase into another. We will be looking specifically at an organic solvent extraction which is a type of solid-liquid extraction. This type of extraction utilizes an organic solvent that has a high affinity for the compound of interest. Most reactions produce a crude mixture of undesired reagent‚ byproducts‚ as well as the
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Edward Cross Organic Chemistry December 10‚ 2012 Title Steam Distillation of Cloves and Extraction of Eugenol Authors Edward J. Cross* Summary The isolation of Eugenol from Cloves begins with whole cloves and then using a steam distillation apparatus to obtain the cloves oil substance. Then with the mixtures of water and dichloromethane to form two immiscible layers. The layers were dichloromethane substance and aqueous The entire substances that are separated into two individual
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Bacteria are the most numerous type of microorganism found in the rhizosphere of the soil. They produce secondary metabolites which are capable of producing antibiotic which eventually inhibit or kill bacteria. The rhizosphere region of the soil is a highly favorable habitat for the proliferation‚ activity and metabolism of numerous microorganisms. The magnitude of this area depends on the plant and the size of the roots that the plant possesses. Bacteria are among the microorganisms living in the
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Bacterial Contamination April 15‚ 2013 Bacteria Contamination The definition of bacterial contamination is food contamination refers to foods that are spoiled or tainted because they either contain microorganisms‚ such as bacteria or parasites‚ or toxic substances that make them unfit for consumption This is very serious and people should take more precaution‚ food contamination is a serious because it results in foodborne diseases that each year affect an estimated seventy-six million
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