Experiment 1: Isolation of Casein from Milk * pH of milk – 6.6 * milk = 87.1% water‚ 4.9 % CHO 3.9 %‚ fats‚ 0.7% minerals Experiment 2: Protein Hydrolysis and Characterization | Reagents | Principle | Test for | Positive Result | Negative Result | Biuret | CuSO4‚ NaOH | Complexation of Cu+2 with amide N atoms | Polypeptide bonds | Violet/purple solution | Blue color solution | Sakaguchi | 10% NaOH‚ 0.02% α-naphtol solution‚ 2% NaOBr | arginine condenses with α-naphtol and NaOH
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Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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Lecture 14 Lecture Gene Complementation in Bacteria In order to perform tests for dominance or for complementation in bacteria we need a way to make the bacteria diploid for part of the chromosome. To do this we need to consider a different extrachromosomal element: Ori T The F plasmid (length 105 base pairs) Tra genes There are some special terms to describe the state of F in a cell: F– refers to a strain without any form of F‚ whereas F+ refers to a strain with an F plasmid. F‚
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important growth factor (vitamin‚ amino acid‚ blood component‚ or carbon source) necessary for the growth of fastidious organisms. Selective Media: Selective media allow for the selection of particular microorganisms that may be present in a mixed culture. Selective media usually contain a component that enhances the growth of the desired organism or inhibits the growth of competing organisms. Differential Media: Differential media allow for the separation of organisms based on some observable
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identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not all of these
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Question: Suppose a meteor impact blows some extremophile bacteria from the Earth into the depths of a gas giant planet called planet X ‚ where they evolve into an intelligent species which practises physics. Do you think their physical theories would be similar to ours‚ and if so in what ways would they be likely to be similar? Your answer will necessarily involve some speculation‚ but make sure it involves some careful argument as well. Theoretical physics is one of many braches of physics that
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Media is a mixed blessing/How ethical is media? Points to be known:- * Media is about all the ways you can get information — books‚ e-media‚ magazines‚ newspapers‚ radio‚ and television. * In present generation media is playing a very important role. * Media is a freedom of speech especially in democratic countries. In Against:- * Media played a big role in getting independence to India as it created awareness in people. * Through media‚ we can know the happenings around
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Cells Now we need to discuss CELLS. You’ve probably heard of cells‚ and may even know of a few‚ such as blood cells‚ nerve cells‚ or skin cells. What are these things called cells? Cells are the smallest living things. You’ll be learning a lot about cells in this course. For now‚ however‚ I’ll introduce you to just a few basic things about them. Cells are extremely tiny organisms that carry out all of the functions of life inside a thin layer of “skin” called (unimaginatively) the CELL MEMBRANE
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Mixed Schools is the optimum Coeducation or mixed-gender education is the integrated education of male and female students in the same environment‚ while unisex education is the education where male and female students attend in separate classes or in separate buildings or schools. In the past‚ mixed schools were not acceptable‚ but by the time it became popular. Mixed schools are better for students socially‚ and it also develops their educational skills and psychological things. Parents recommend
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It has a physical significance‚ as well as an emotional significance. It seems to find a part in the life of everyone in the community. There are many situations in the story where the boy feels separated and detached from Mangan’s sister‚ his love. His feelings for her are so strong that he feels he needs to isolate himself in order to keep her out of reach. Even though they barley communicate‚ the mere image of her brings him much happiness. As the boy illustrates
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