In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
Premium Ethanol Enzyme Chromatography
Isolation and Characterization of Maize Acid Phosphatase | BCHM 405 | Aaron BANESEH 10307275 | Table of Contents 1 Table of Contents 2 2 Introduction 4 3 Experimental Procedures 5 3.1 Sample preparation 5 3.2 Initial Extraction of Acid Phosphatase 5 3.3 Determination of Protein Concentration by the Folin-Lowry Assay 5 3.4 Specific Activity Assay 6 3.4.1 Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of
Premium Enzyme PH
forces that practically drown out the boys’ good intentions and extract the boys from living in a fulfilling life as a child in society within Lord of the Flies by the author William Golding seems to always be discussed first rather than talk about what little but meaningful the good forces are that remain on the island. For example‚ Jack Merridew and his tribe’s savagery is regardless of where you find source from‚ rather it be your teacher or a website like Sparknotes is usually saying the same
Premium William Golding
The plotting subconscious fabricated their warmth around; he drew comfort from the illusion that he was not alone. He playfully rolled against the floor (filled with reckless joy‚ wondering how amused they were by his childish display of happiness) his ears smudged against the cold tiling and picked up the fractured noises of the midnight revelry in the apartment below. He denied it at first‚ the sounds were coming from his house‚ but its muffled nature struck him with the truth. Nothing but a
Premium Figure 8 Temperature 2006 albums
provides a surface for the individual cells to be separated and isolated from the other microbial cells that may be present in the original sample. C. Compare your L. acidophilus pour plates and spread plate. Which method do you think worked better to isolate individual colonies? Why? I think spread plates worked best because a solid medium that provides a surface for the individual cells to be separated and isolated from the other microbial cells that may be present in the original sample
Premium Management United States Strategic management
Photosynthetic Activity in Plants Using Different Wavelengths of Light using Chromatography and a Spectrophotometer In this experiment‚ the separation of particular pigments‚ contained within a fresh spinach leaf‚ were examined. Paper chromatography is the process of separating certain molecules‚ or pigment molecules‚ based upon their polarity. Four different pigments were examined from the spinach leaf: chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and carotene. Based upon looking at the pigments individual
Premium Visible spectrum Electromagnetic radiation Light
Freak the Mighty Final Different‚ isolated‚ excluded... all of these words describe Max’s room. It’s different from his grandparent’s room because it’s a cellar‚ it’s isolated from their room‚ and excluded from the house. Strangely enough‚ Max feels this way about himself as well. Throughout the entire story Max refers to himself as a “total goon” which tells the reader that he thinks of himself as dumb person and he feels different. He knows he’s not as smart as everyone else and when he’s put
Premium
Loneliness and Isolation in Jane Eyre In Charlotte Bronte’s novel “Jane Eyre”‚ the eponymous protagonist suffers throughout the plot from loneliness and isolation‚ and these two themes interweave for the duration. Jane’s loneliness and isolation are repeatedly linked to her “physical inferiority”‚ and this phrase is used by Jane to describe herself very early on in the novel. Jane is small‚ underdeveloped‚ pale and timid‚ which often means she finds herself helpless and different to everybody
Premium Jane Eyre
Social isolation is the separating of individuals or groups of people from a greater group for various reasons. Social isolation can be voluntary‚ involuntary‚ and even executed covertly without the isolated party being privy. Voluntary isolation spans from a leave of absence at work to entering a drug or alcohol rehabilitation center or attending college. It can result from a desire to fulfill life long dreams such as traveling the world or be the produced result of discontentment with ones life
Premium
The theme of isolation is the one theme that is most developed thoroughly throughout Mary Shelley’s novel. From the beginning of the novel someone has felt isolated from someone else. Victor‚ the creature‚ and Elizabeth are three characters that have most developed the theme of isolation. Victor develops the theme of isolation in the novel. Unlike the creature and Elizabeth victor chooses to isolate himself. While working on his research and trying to create life victor loses all contact with
Premium Frankenstein English-language films Mary Shelley