Strawberry DNA lab Extraction Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments. Background information: The long thick fibers of DNA store the information for the function of the chemistry
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DNA Isolation from Onion! Today‚ the students are going to isolate DNA from onions. You will need a blender or a food processor for this experiment. If you do not have one‚ check to see if your teacher does. If neither of you have one‚ then let me know ahead of time‚ and I can provide one from my home. You will also need to bring a knife and cutting board to cut the onions as well as a measuring cup. All other materials will be provided. The first part of the DNA experiment is to grind
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Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells
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EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
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The hypothesis for this lab is if strawberry DNA is separated from other components‚ then when it is placed in a insoluble solution the DNA can eventually be isolated. DNA is deoxyribonucleic acid. Its a self replacing material present in all living organisms as the main constituent of chromosomes. It is the fundamental and distinctive characteristics of someone or something‚ especially when regarded as unchangeable. DNA extraction is the removal of deoxyribonucleic acid from the cells or viruses in
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extract DNA from a strawberry using limited household items. This was accomplished with the prior knowledge that the DNA is stored in the nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants
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purpose of this lab was to isolate DNA from a food sample‚ amplify the DNA using a polymerase chain reaction‚ and test the amplified DNA for the presence of the Bt gene or the 35s promoter. In part one of DNA isolation‚ the food sample was crushed before Lysis Buffer was added‚ in part to break down some cell walls‚ but also to increase area of the food sample being touched by the Lysis Buffer. The purpose of Lysis Buffer is to break down the cells in the food sample and release their DNA into the solution
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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