Procedures: In the first lab‚ seven test tubes were attained and six of them were filled with the solutions that were listed (Na Pyruvate‚ MgSO4‚ NaF‚ Glucose‚ Water‚ and yeast suspension). The last test tube was filled with water. After they were filled with the solutions they were incubated at 37 degrees Celsius for about forty minutes. After the forty minutes passed take the test tubes and measure the height of the bubbles that formed in millimeters. For the second lab‚ attain three beakers‚
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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in Sordaria 979554296 Biology 110 Lab Introduction: In Israel there exists multiple spots in the mountains called Evolution Canyons‚ which are all located between a southern facing slope (SFS) and a northern facing slope (NFS). What’s particularly interesting about these locations is that despite the two slopes being on opposite sides of a small canyon‚ they exhibit extremely contrasting conditions. The SFS receives multiple times the UV radiation from the sun that the NFS receives‚ has fluctuating
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Egg Lab Report Introduction: An egg is a model of a human because the egg has a cell membrane like humans do inside and outside of the body that let things pass through like water. We can use eggs to study the effect of changes in the external environment on the internal environment by having harsh environments like putting the egg in only alcohol and see what happens to the inside of the egg. Diffusion is the movement of a substance down its concentration gradient from a more to a less concentrated
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to take DNA from the Halobacteria strains and examine their genotypes. The PCR reaction’s purpose was to locate specific
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are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
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this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps in going from reactants
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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Abstract An attitude is a representation of an individual ’s degree of like or dislike for an act‚ object or evaluation. This report aims to conclude whether there is a difference between female and male attitudes towards smoking. It is hypothesised that females will have a more positive attitude towards smoking. Questionnaires using a 5 point likert scale will be utilised to measure each participants’ attitude towards smoking through their responses. Results stated there is no significant difference
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