Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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Extraction of Eugenol from Cloves 02/01/2014 CHM2210L University of North Florida Abstract Eugenol is found in the essential oil of cloves and has distinct properties that make it an important product to both food and drug industries. (Bhimrao‚ et. al‚ 2004). To utilize many of eugenol’s characteristic properties‚ it is necessary to isolate this organic chemical from the other components of cloves. In this experiment‚ eugenol was isolated from a sample of cloves using a series of techniques
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Leah Romero 04/18/2018 Lab 9 Conclusion Chem. 102L For lab 9‚ isolation of casein‚ the main purpose was to take the milk sample that was provided in the lab and through the addition of acid‚ be able to find the pH of the milk at the isoelectric point of casein. Isoelectric point is the pH at which a particular molecule carries no net electrical charge. The main points of the lab or the outcomes we were looking for were to be able to look at the properties of proteins in casein and be able to identify
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Isolation of Eugenol from Cloves by Steam Distillation and its Identification by Infrared Spectroscopy Eim A. Chemist CHEM 303 June 16‚ 2005 INTRODUCTION “Essential oils” are the volatile components associated with the aromas of many plants.1 In this experiment‚ the essential oil eugenol (the main component of oil of cloves) will be isolated from ground cloves using the technique of steam distillation‚ which is often used to isolate liquid natural products from plants.2 The principle
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Essential oils are volatile components associated with the aromas of seeds‚ bark‚ or roots of plants. In this experiment‚ the essential oil eugenol was isolated from ground cloves using steam distillation techniques. Eugenol had a high boiling point‚ but it was isolated at a lower temperature by performing a co-distillation with water‚ a process known as steam distillation. This technique allowed the desired material to be distilled at a lower temperature than 100℃. The point of the steam distillation
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Chemistry 2423‚ Section 304 25 October 2017 Lab 6: Natural Products I The main goal of this experiment was to employ isolation techniques to derive chemicals from two different natural sources and study their properties (Clement 91). The two natural sources used in this experiment were an orange (the peel contains limonene‚ which is a terpene) and a dichloromethane solution of clove oil (which contains eugenol‚ an acetogenin). To isolate the eugenol‚ 20 mL of dichloromethane solution of clove
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LAB Report #3 Introduction: In this lab we have focus on Isolation of bacteria from environment. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes‚ walls‚ furniture; in soil and dust; and on and in our own bodies (skin and mucous membranes). In order to demonstrate the ubiquity and diversity of microbes in the environment‚ samples from immediate areas of the environment and/or from your body will be obtained and cultured
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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