Experiment 1: Isolation of Casein from Milk * pH of milk – 6.6 * milk = 87.1% water‚ 4.9 % CHO 3.9 %‚ fats‚ 0.7% minerals Experiment 2: Protein Hydrolysis and Characterization | Reagents | Principle | Test for | Positive Result | Negative Result | Biuret | CuSO4‚ NaOH | Complexation of Cu+2 with amide N atoms | Polypeptide bonds | Violet/purple solution | Blue color solution | Sakaguchi | 10% NaOH‚ 0.02% α-naphtol solution‚ 2% NaOBr | arginine condenses with α-naphtol and NaOH
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Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial
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Isolation in light and shadow The painting “New York Movie” is an incredible painting with significant use of light and shadow and the contrast of warm and cool colors. It’s not just a painting of a nicely dressed lady in a theater. It’s very intense and strong. Edward Hopper defines the isolation of urbanites by the design of this painting. The arrangements of figures and settings capture the moment of “not belonging”. By the composition‚ contrast of colors and the use of light and shadow‚
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Organic Lab 1: Fractional Distillation Discussion: With the purpose of the experiment being to identify the 30 mL of unknown liquid‚ the theoretical basis of simple and fractional distillation must be deconstructed and applied to the data obtained describing the liquid in question. Simple distillation is a separation technique which can be used to separate and purify distillates from a liquid mixture which ideally contains one volatile and one non-volatile compound. If such ideal conditions are
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Simple Distillation Objective The purpose of doing this experiment is to separate two miscible liquids samples of cyclohexane and toluene using simple distillation. The objectives will be to record boiling range and volumes (mL) of distillates that are attained during the process of distillation. Background Distillation is a frequently used technique for separating mixtures based on differences in the boiling points of components in the mixture. The product obtained by distillation‚ is termed
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Many gigantic companies nowadays apply social media in business for marketing and advertising. Through media the business easily reach the customer easily. For example‚ like today‚ when customer interact with the companies and brand incessantly through mobile apps‚ and social media it show more effective than manual service. This is because‚ through social media the company can up dated their business through interesting picture and marketing. Customer can easily keep up with the company business easily
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ethanol. The solution is then cooled slowly‚ and the aspirin crystallises out of solution leaving the salicylic acid and other impurities behind. In my experiment‚ pure aspirin was obtained after filtering out the impurities and excess reagents through the filter paper. A method to check a solid compound’s purity after recrsytallisation is to check its melting point. The melting point of a compound can be used to identify it and also to estimate its
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Beloved was a famous novel written by Toni Morrison. In the novel‚ Morrison narrated the strange things that happened at 124 Bluestone Road and the life of a former slave‚ Sethe. Sethe had four children but her daughter died from a throat cut before she escaped to Ohio. When she arrived in Cincinnati‚ Ohio‚ she lived in a haunted house with her daughter‚ Denver‚ her two sons‚ and her mother-in-law‚ Baby Suggs. However‚ by 1873‚ Sethe and Denver were the only people living in the house because Baby
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The process of distillation is commonly used to purify liquids. The process of distillation allows separation of distillates based on the temperatures at which they boil. Liquids with higher volatility boil at lower rates than liquids that are less volatile. Because of this when a mixture of two substances is created there will be a spike in the boiling point. At the spike the more volatile substance has vaporized and condense out of the mixture leaving the less volatile liquid. Thus the temperature
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Experiment 2: Dehydration of an Alcohol: Distillation and Gas Chromatography Preparation of Methylcyclohexenes Purpose: The basic purpose of this experiment is to carry out the dehydration of an alcohol and isolate the reaction products by distillation. Gas Chromatography will be utilized to analyze the reaction mixture. Table of Reagents: Compound (g) Molecular Weight (g/mol) Grams (g) Moles 6 mL of 2-methylcyclohexanol (C7H14O) 114.19 g/mol 6 mL x 0.943g = 5.66 g 1 mL 5.66 g x
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