separate individual cells of a particular microbe. This requires the use of a solid medium that provides a surface for the individual cells to be separated and isolated from the other microbial cells that may be present in the original sample. C. Compare your L. acidophilus pour plates and spread plate. Which method do you think worked better to isolate individual colonies? Why? I think spread plates worked best because a solid medium that provides a surface for the individual cells to
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Leah Romero 04/18/2018 Lab 9 Conclusion Chem. 102L For lab 9‚ isolation of casein‚ the main purpose was to take the milk sample that was provided in the lab and through the addition of acid‚ be able to find the pH of the milk at the isoelectric point of casein. Isoelectric point is the pH at which a particular molecule carries no net electrical charge. The main points of the lab or the outcomes we were looking for were to be able to look at the properties of proteins in casein and be able to identify
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Isolation of Caffeine from Tea (12A) Introduction When a person drinks tea or coffee‚ it is not pure caffeine. It also has other natural substances. The purpose of this lab was to isolate caffeine from tea leaves. The methylene chloride removed nearly pure caffeine from the basic tea solution. Caffeine is a white‚ crystalline‚ bitter alkaloid‚ C8H10N4O2 ‚ usually derived from coffee or tea. Caffeine is used in medicine chiefly as a nervous system stimulant. Caffeine is used by humans to ward off
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In brief‚ the purpose of this lab was to isolate DNA from a food sample‚ amplify the DNA using a polymerase chain reaction‚ and test the amplified DNA for the presence of the Bt gene or the 35s promoter. In part one of DNA isolation‚ the food sample was crushed before Lysis Buffer was added‚ in part to break down some cell walls‚ but also to increase area of the food sample being touched by the Lysis Buffer. The purpose of Lysis Buffer is to break down the cells in the food sample and release their
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ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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To My parents‚ Mr. Therdsak and Mrs. Aree Jainonthee who always behind every success of my life ACKNOWLEDGEMENT I would like to express my sincere thanks to my thesis advisor‚ Asst. Prof. Dr. Duangporn Pichpol for her invaluable suggestions and encouragement throughout the period of this research. I am most grateful for her teaching and advice on research methodologies and techniques performed in microbiology testing. Her close attention on my working life and my career path are very much appreciated
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Jolyne Piet CHM-221L-02 Lab #2: Experimental Design Isolation of Sucrose: 3.01 g Panacetin were weighed in a 125-mL Erlenmeyer flask‚ and 51mL dichloromethane were added to partially dissolve the Panacetin. The insoluble portion was gravity filtered and air dried to yield 0.45 g of sucrose (15.0 % of original Panacetin). Isolation of Aspirin: The organic filtrate was extracted through a separatory funnel with 32 mL 5% sodium bicarbonate to produce an aqueous layer and a dichloromethane layer
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Isolation‚ Morphological‚ and Biochemical Characterization. A high-throughput screening of cartenoid-producing microorganisms resulted in 25 astaxanthin-producing bacterial strains from marine water samples that were collected from the Pacific coast of Japan (unpublished results). Among the isolates‚ a novel‚ highly selective astaxanthin-producing marine bacterium‚ labeled strain (N-5)‚ was isolated based on its ability to produce orange pigment on NA plate after 2 days of cultivation at 30 °C. Strain
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