Bromination Solo Experiment 3 – Individual Lab Report (Save as pdf and submit‚ due by 12:00 NOON one week after experiment) Last Name: First Name: TA Name: Date Lab Performed: Date Lab Submitted: Group A‚ B‚ or C: Comments for Grading TA: (Please indicate if you performed the lab on a day other than your regularly scheduled day and/or with a TA other than your regular TA). Page Limit: report must not exceed FIVE pages (including this page) LIMIT DOES NOT INCLUDE ANY GRAPHS‚ SPECTRA
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Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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Running Head:ISOLATION 1 ISOLATION A LONELY ROAD Elizabeth Arguelles Keiser University ISOLATION 2 ISOLATION A LONELY ROAD There is a difference between the unhealthful experiences of isolation from the healthful and necessary experience of occasional solitude. Having time alone is an important part of emotional maturity. Sometimes we need alone time to sort through are thoughts‚ distress from a busy day. In a demanding world of seven billion people‚ restore a connection with
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Katherine Waide Mr. Feliciani Honors English 1 Period 4 27 September 2016 Isolation The fiction novel‚ Of Mice and Men‚ written by John Steinbeck follows the captivating story of two migrant workers. The book takes place in the heat of the Great Depression when finding a job was extremely difficult. During this era‚ many people became migrants‚ traveling from farm to farm picking fruit and other crops‚ barely earning enough money to escape starvation. The lack of food‚ clothing‚ shelter‚ and
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Isolation Have you ever felt isolated from your surroundings‚ either because of language or culture wise‚ being new in town‚ feeling friendless‚ or simply because you have to study while everyone else is out having fun while you study? Well I have had many experiences like those. There was a time in which I had moved from New Jersey to Tennessee and I was the new kid on the block. Also there was another addition in my life
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were two colonies with England settlers‚ these colonies were very different. New England economy was base on growing crops and livestock‚ unlike the Chesapeake who depended greatly on the king of England for economic support. The New England colony who came to the new world for religious freedom practiced Christianity. On the other hand Chesapeake colony was mostly from the Anglelican church who at the time were actually a ruling government and religion was not important. Although both colonies would
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Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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Geography was the primary factor and played an important role in shaping the development of the British colonies in North America. In some areas‚ the geography influenced the living and farming conditions for the better‚ and for some areas‚ for the worse. The Southern colonies’ geography was well-suited to farming. It was warm year round and provided a great place to produce cotton‚ indigo‚ rice‚ and many other crops. However they had few natural harbors. Opposite the North had thin rocky soil
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Establishing the Colonies Name _____ Motives for settling in the New World: Spread Christianity Find a short cut to Asia Better job opportunities Roanoke Island With the permission of Queen Elizabeth‚ Sir Walter Raleigh raised money to establish a colony‚ and in 1585 a small group of men sailed for the Americas. What did Raleigh find when he returned to the Lost Colony of Roanoke in 1589? The people had vanished and they found the word‚ “Croatian” carved in a tree. Jamestown What
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