kingdom of microorganisms was brought into life 3 billion years ago. Since then «the earth is a fundamentally a microbial planet‚ to which the micro-organisms are recent the relatively unimportant additions» (Wheelis‚ Mark‚ Principles of modern microbiology‚ 2008‚ p.1). The term microorganisms consist of bacteria‚ archaea‚ fungi‚ and protest‚ which can either be unicellular or multicellular. They first have been studied by Anton van Leeuwenhoek by the use of his own design microscope‚ in 1675. However
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Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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Increasing evidence suggests that the alarming rise in allergic and autoimmune disorders during the past few decades is at least partly attributable to our lack of exposure to microorganisms that once covered our food and us. As nature’s blanket‚ the potentially pathogenic and benign microorganisms associated with the dirt that once covered every aspect of our preindustrial day guaranteed a time-honored co-evolutionary process that established “normal” background levels and kept our bodies from overreacting
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Open Reading Frames * (ORFs) are regions with no stop codons. All genes reside in long open reading frames * Note that stop codons in other reading frames have no effect on the gene. * Can be searched in the genome sequence. Valid only for prokaryotes and lower eukaryotes. Protein Sequencing vs. DNA sequencing * We compare protein sequences‚ not DNA‚ because protein is more conserved in evolution than DNA * The organism’s survival depends on the protein being
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Chapter 1: The Main Themes of Microbiology What is Microbiology? • micron = small and biologia = study of living things • Microbiology = study of small living things • How small is “small”? • What do we really mean by living? • Working definition: Study of entities too small to be seen with the unaided human eye. • (< 0.2 mm = 200 µm = 200‚000 nm) Types of Microbes Prokaryotes include Bacteria and Archaea Eukaryotes include Protista (Algae and Protozoa)‚ Fungi‚ and Animalia
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7351_2006_1_S03 Rajiv Gandhi University of Health Sciences‚ Karnataka PM.D. [MICROBIOLOGY] DEGREE EXAMINATION Time: 3 Hours [Max. Marks : 100] PAPER I – GENERAL MICROBIOLOGY AND IMMUNOLOGY QP Code: 7351 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. Answer all questions LONG ESSAYS 1. 2. 2 X 20 = 40 Marks Discuss the production‚ properties and uses of monoclonal antibodies Discuss various in vitro antibiotic sensitivity
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SCOPE OF MICROBIOLOGY: What is Microbiology? | � Microbiology is the study of microorganisms� Microorganisms‚ roughly‚ are living things that are too small to be seen with the naked eye� Microorganisms cannot be distinguished phylogenetically from �macroorganisms� (see variety of microorganisms‚ below)� Microbiology is more a collection of techniques: aseptic technique‚ pure culture technique‚ microscopic observation of whole organisms‚ etc.� Microbiologists
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Chapter 1. Microbiology – Diversity of Organisms Microorganisms- too small to be seen with the unaided eye “germ”- rapidly growing cell Microbes in our lives Pathogenic- disease causing Decompose organic waste Producers in ecosystem (photosynthesis) Produce industrial chemicals such as ethanol and acetone Produce fermented foods ( vinegar‚ cheese‚ bread) Produce products used in manufacturing (cellulose) and treatment (insulin) Designer Jeans: Made by Microbes? Stone washing- Tricoderma
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Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial
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allergen or a contamination outbreak. This analysis was done by utilizing all of the proper methods taught thus far in the microbiology laboratory for the identification of two unknown bacteria. An unknown labeled as number 3 was given out by the lab instructor. The methods that have been lectured and absorbed pertaining to identifying
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