Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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This essay will compare and contrast the feeling of isolation faced by the characters in Marilyn Chin’s Moon and Murakami Haruki’s Super-frog Saves Tokyo. The way they adopted to regain connection to the society and to reduce the feeling of loneliness and isolation would also be examined. A comparison would also be made between isolation caused by inter-ethnic factors and intra-ethnic factors. Moon was the first story of Marilyn Chin’s book‚ Revenge of the Mooncake Vixen. It was a Chinese American
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English Controlled Assessment Question: Explore the methods Steinbeck uses to demonstrate the effects of isolation on people in Of Mice and Men “You’re all scared of each other that’s what.” This quote is one of the main points of John Steinbeck’s novel‚ Of Mice And Men‚ the effect of isolation on human beings‚ and specifically the culture of our nations that forces people to be isolated. Steinbeck almost immediately sets the course of the book with his setting‚ the 1930’s depression was an
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The movie‚ Gran Torino‚ is about an old war veteran who has just lost his wife. He is alienated from his family and lives alone with his dog. He suffers from a terminal illness but doesn’t tell anyone. When one of his neighbors‚ Tao‚ is pressured into trying to steal Walt’s Gran Torino he gets caught by Walt‚ and Tao’s mother makes him work for Walt in order to make up for trying to steal his car. The more Tao works for Walt‚ the more they begin to respect each other. Eventually‚ Walt helps Tao become
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Anise oil was successfully able to be extracted from anise seeds during this experiment. It was done by first crushing the seeds‚ then placing them into a distillation apparatus. The distillation apparatus evaporated the liquid from the seeds‚ which was captured in a flask. Next‚ DCM was added to the distillate‚ which allowed the water to be separated from the anise oil. Finally‚ the DCM evaporated and the pure anise oil was collected. The process of steam distillation allowed the anise oil in
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Isolation of Trimyristin from Nutmeg and preparation of Myristic Acid from Trimyristin by Hydrolysis Introduction Over 40% of medicinal chemicals in the world were developed from isolation of the natural source. These sources were from fungi‚ plants‚ bacteria‚ animals‚ etc. The techniques used to isolate and purify has been by extraction‚ distillation‚ recrystallization‚ and chromatography. In this experiment we will be trying to obtain a pure organic compound from a natural source. Because this
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How does John Steinbeck portray loneliness and isolation in the novel “Of Mice and Men”? The novel of “Of Mice and Men” was written in the time of the great depression in the 1930’s in America‚ this was the time in which Professional workers became Migrant workers due to the Wall Street crash in 1929. The great depression caused many professional workers to turn to working in farmland harvesting wheat. John Steinbeck published the novel of “Of Mice and Men” in 1937 it is set in Salinas‚ California
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E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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INTRODUCTION Cell isolation and culture are essential tools for the life sciences today specifically in the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution under the microscope‚ that is not possible in whole animals or even tissue explants (Zhang and Kuhn‚ 2011). In its simplest form‚ cell culture involves the dispersal of cells in an artificial environment composed of nutrient solutions‚ a suitable surface to support
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Effects of loneliness and isolation in the short stories “Metamorphosis” and “The Yellow Wallpaper” In Franz Kafka’s “Metamorphosis” and Charlotte Perkins Gilman’s “The Yellow Wallpaper‚” the reader can easily see demonstrated the effects of the alienation and loneliness both of the main characters experience. The feeling of loneliness and the state of alienation leave the characters changed from who they were and manages to completely
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