The Urea test was used to test both Unknown Gram-positive and Unknown Gram-negative bacteria. One colony of bacteria was used from the Unknown 13A plate and Unknown 13B plate in order to inoculate the Urea Slant. After the slant was inoculated‚ the cultures were placed in an incubator at 35ºC for Gram-positive bacteria‚ and 37ºC for Gram-negative bacteria for a total of 48 hours. If the Urea test were positive‚ the color of the slant would have changed to a hot pink color‚ which indicated the bacteria’s
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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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However‚ the mucoadhesion strength of AI5 and AI20 beads was significantly higher than the alginate beads and reached zero after 45 min and 120 min for the IA20 and IA5 formulas‚ respectively. Similar to jejunal mucosa‚ AI20 beads were completely dissolved at 45 minutes. Between 5 and 45 min no significant differences were observed between AI5 and AI20. Overall‚ mucoadhesion strength decreases faster for jejunal mucosa than for colonic one. This can be explained by the thickness of the mucin layer
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amounts of glucose‚ amino acids‚ urea‚ and salts in urine. To test this we used different chemicals that would react with one of the four items above showing either a high concentration or low concentration within the urine. By using a solution called silver nitrate we could test whether or not salt was present in the urine. If the urine turned a cloudy white after adding the silver nitrate the urine had a high concentration of salt. To test for amino acid we used a chemical called biuret solution. If
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Food Test 1: Test for Glucose – with Benedicts solution Benedicts solution is used to test for simple sugars‚ such as glucose. It is a clear blue solution of sodium and copper salts. In presence of simple sugars‚ the blue solution changes color to either green‚ yellow or brick-red‚ depending on the amount of sugar. Method 1) Mix smalls amount of each food sample (i.e.‚ Egg lumen‚ cylindrical piece of potato tuber‚ bread crump and crisps) in different test tubes with distilled water to make a
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and highly reactive metals. Alkaline earth metals on the other hand‚ comprise the group 2 elements. They are all shiny and silvery-white on appearance and they are somewhat reactive metals. It is said earlier that a metal can be found in a compound. And in order to detect the presence of metals in a compound‚ flame test is done. The result of the flame test is based on each element’s characteristic which is emission spectrum. Flame test involves placing a sample of an element or compound in a
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> # Ho’s Maple Lab Test Solution: Semester 1 2012 (1) # Question 1; > evalf(100*sin(95)‚38); 68.326171473612098369957981656827095404 > # Queston 2; > f:=x->3*sin(1/4*x^4)-sin(3/4*x)^4; (2) > # Find 1st derivative; > D(f); (3) > # Find turning/stationary point in the interval [1‚2]‚ 1st derivative expression = 0‚ 10 significant figures!; > evalf(fsolve(3*cos((1/4)*x^4)*x^3-3*sin((3/4)*x)^3*cos((3/4)*x)= 0‚x=1..2)‚10); 1.562756908 (4) > # Find 2nd derivative at x= 1.562756908; 10 significant
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efficiency. The two variables that we tested were: molar ratio of insert (RFP): vector (P1 plasmid)‚ as well as the effect of removing the stuffer from the digested plasmid. We predicted that the cells transformed without stuffer would have higher ligation efficiency than the cells transformed with stuffer. The reason for this is because the stuffer (if not removed) would cause the plasmid to ligate back into itself
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green sparks also. Both of the sodium bottles turned the flame orange for a short time. Electrons orbit the nucleus of an atom in energy levels. If atoms are heated up to the point where the electrons are orbiting the atom shells at a faster pace than usual‚ the energy from the electrons when they are slowing down their pace is given off in light form‚ and when the energy levels are back to normal again‚ the colour of the flame is back to the luminous blue light. Different atoms give off different
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cocci shaped. Some sections of the smear were too concentrated‚ which was hard to identify the shape of the bacteria‚ while other sections were less concentrated and was easier to analyze the shape. The next step according to the result‚ will be a catalase test.
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