Protein Concentration using BCA and Bradford Assays Introduction There are several ways to determine protein concentration for a sample such as The Lowry Method‚ Bradford assay‚ and Bicinchoninic Acid (BCA) just to name a few. Determining the protein concentration in your sample is an important step in any laboratory workflow that involves protein extraction and/or analysis (1). Based on the assay being used‚ different chemicals can alter the accuracy for the results. For example‚ in BCA‚ the reducing
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When Thomas Bradford woke up that morning‚ he realizes that the bed was too small for him because part of his legs were outside of it‚ but his hands were as small as always. He looked through the big window of his big room and he realizes that it was still dark outside and very cold‚ he was surprised because it was summer time and every day before this one had been sunny and hot. How could this be? He asked to himself. He had spent his whole life working since he was a kid just to build an empire
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Johnny Williams‚ who is a jazz percussionist and his brother‚ Jerry‚ who is also a percussionist John wouldn’t be the John Williams we all know today. In his early age‚ he studied in North Hollywood High School graduating in 1950. He later went to the University of California‚ Los Angeles‚ and concentrated deeply with his music with the Italian arranger Mario Castelnuovo-Tedesco. He‚ then‚ met many people when we were working with Steven Spielberg on the recording of Star Wars (King B3). Williams grew
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in 1792 by Henry Walton Smith and his wife Anna in Little Grosvenor Street‚ London. WH Smith currently operates over 1200 stores‚ primarily in UK. It sells a wide range of products‚ which are divided into four categories: stationary‚ books and impulse as well as small range of entertainment products. Mission Statement: “WH Smith aim is to be Britain’s most popular book seller‚ stationer and newsagent”. Getting into more details of this statement it seems clear that WH Smith reached their purpose and
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Will Smith In my life ‚ I have had many Idols-From Eddie Murphy to Tupac‚ they have never been scarce in number. However‚ I am taking this time to dedicate my essay to the one person I find has had an adventurous life-Will Smith. At the age of sixteen‚ Smith met a DJ at a party. The pair became friends‚ and the rapping duo DJ Jazzy Jeff and soon enough the Fresh Prince was born. Wilfard Christopher Smith Jr. was
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what is already there. Will Smith is an actor but have you always wondered why he wanted to be an actor. Willard Christopher Smith‚ Jr was not always an actor but merely a rapper. Yes its true he was not always an actor he was a rapper for some of his life. If you want to Find out read my Biography. Throughout life people will make you mad‚ disrespect you and treat you bad. Let God deal with the things they do‚ cause hate in your heart will consume you too. Will Smith was born on September 25‚ 1968
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John Muir and William Wordsworth Have you ever been or seen something that makes you truly blissfully happy? Maybe gone on a speedy roller coaster. Going up and down at lightning speed‚ the adrenaline coursing through your veins. In those few minutes having no worries only feeling as free as a bird. That is how John Muir in the essay The Calypso Borealis and William Wordsworth in the poem I Wandered Lonely as a Cloud felt about nature. They use vivid writing to describe how freeing their experiences
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English 1 5/4/15 Comparing essay and poetry The two authors John Muir and William Wordsworth are two authors that write two different types of literature‚ one being poetry and the other being essays. These two illustrative literature artists both included nature in their writings. They say that poetry and essays are completely different but on the other hand they have similarities. In the essay "Calypso Borealis" written by John Muir he compared his life and his feelings to the world around him
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During this experiment‚ the method of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was conducted using a negatively charged protein and pre-stained molecular weight markers. The hypothesis was that the molecular weight of N-acetyl-β-D-hexosaminidase B would be 28‚000 kDa. To confirm or reject the hypothesis‚ the molecular weight of N-acetyl-β-D-hexosaminidase B and the concentration of protein had to be determined. The electrophoresis of the protein gel were conducted using
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Smith Consulting has been retained in order to develop a functional and effective Frequent Shopper program for Kudler Fine Foods. Kudler Fine Foods has decided that it is in its best interest to implement this type of new marketing plan in order to build customer loyalty. The program will allow the shoppers at Kudler Fine Foods to earn points based on the purchases they make. These points will allow consumers to earn high end rewards. Kudler Fine Foods has specified to Smith Consulting that the desired
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