Chemistry 121 Experiment 19 Molar Mass Determination y Depression of the Freezing Point Introduction: The most commonly used liquid is water. In this experiment we study the equilibria that can exist between pure water and an aqueous solution‚ and ice‚ the solid form of water. The heat will transfer from a higher temperature to a lower temperature. In order for water to change states of matter‚ it takes a certain amount of kinetic energy or heat. The shift from ice to water (solid to a liquid)
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Osmosis/Plasmolysis Lab Name: Problem: How do solutions of various salt concentrations influence osmosis in relation to an onion cell? Materials: (per student group): red onion epidermis forceps‚ dropper distilled water NaCl solution paper towels microscope slide cover slip Procedure 1. Make a wet mount of the red onion epidermis. 2. Examine under low power. When you have a clear view of several cells‚ switch to high power. Make a labeled drawing
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gain (g) because the solution is hypotonic – a hypotonic solution results in the water diffusing into the cell because the molecules of the substance are moving from an area of higher concentration (outside of the cell) to an area of lower concentration (inside the cell) (Hammel & Schelegel 2005). Therefore‚ the water was moving into the cell‚ causing the potato cell to increase in size. The potato appeared to be quite turgid and swollen through observation. The 3% salt solution resulted in the least
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Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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cells‚ we must understand tonicity as the ability of a nearby solution to cause a cell to lose or gain water‚ depending on its concentration of non-penetrating solutes relative to solutes inside the cell (Reece et al. 133). The dialysis bags used in this experiment have membranes which are selectively permeable‚ which only allows particles specifically small enough to pass through (Carmichael‚ Grabe and Wenger). In a hypotonic solution‚ water
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Law‚ which is the linear relationship between absorbance and concentration of an absorbing species. Absorbance formula is shown in fig. 1.1. However‚ the Beer-Lambert Law is not obeyed at high concentration as solution with high concentration may alter the refractive index of the solution which in turn may affect the absorbance reading. The limitation of the Beer-Lambert Law is shown by the non-linear part of the graph in fig 1.2. The absorbance
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procedures each chemist must master to become proficient in the laboratory. Crystallization is based on the principles of solubility: compounds (solutes) tend to be more soluble in hot liquids (solvents) than they are in cold liquids. If a saturated hot solution is allowed to cool‚ the solute is no longer soluble in the solvent and forms crystals of pure compound. Impurities are excluded from the growing crystals and the pure solid crystals can be separated from the dissolved impurities by filtration. (orgchem
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lecture‚ you will be able to Define viscosity and viscosity coefficient Outline the method to measure viscosity using Ostwald viscometer Determine the average molecular weight of a polymer Determine the surface concentration of 1-butanol in aqueous solution Measure the distribution coefficient of a solute betweenn two solvents 39.1 Introduction Viscosity‚ one of the transport properties‚ arises because of intermolecular attractive and relatively long-range forces. Viscosity coefficient ([pic])
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Research Question: Which salt solution caused the sailor to die? Hypothesis: If the sailor drank the 40% salt water solution‚ then would die sooner‚ because the high amount of salt causes more dehydration. Variables: Independent- Salt Solution Dependent- Mass of Potato Control- Tap Water Materials: •Tap Water (50 ml for each cup) •Salt •Beaker •3 cups •3 pieces of potato •Scale Procedure: Step 1: Gather Materials. Step 2: Weigh each potato separately on the scale and record weight
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during this process‚ water moves down its concentration gradient. A solution surrounding a cell is hypertonic if it contains more solute particles than the inside of the cell‚ and the water will move out of the cell into the surrounding hypertonic solution by osmosis. If the solution is hypotonic (containing fewer solute particles than the inside of the cell)‚ the water will move from the solution into the cell. When the solution is isotonic (the same concentration of solutes as the cell)‚ the cell
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