sterile condition and homogenized using base of a syringe in PBS. Then‚ the resuspansed solution was washed twice in 300g centrifuged for 10min. erythrocytes were lysed Using cold Ammonium-Chloride-Potassium (ACK) lysis buffer (0.15 M NH4Cl‚ 10 mM KHCO3 and 0.1 mM Na2EDTA) for 5 mints at room temperature. Lymphocytes were washed with PBS contained 2% FBS and resuspened in RPMI-1640 supplemented with 10% FBS (Gibco‚ BRL‚ Maryland‚ USA). Viability and count of the cells was evaluated with aid of 0
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Introduction Forensic entomology is used to determine such crimes as murder‚ suicide‚ and other criminal acts by examining various insects instead of using human tissues. This forensic tool is used to determine the postmortem interval of a corpse and the cause of death of a corpse when all other forms of human evidence (human blood‚ tissues‚ hair‚ etc.) are not present at the scene. Forensic entomologists prefer using insects to determine these factors of death because the insects produce similar
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CHEMICAL REACTION (SYNTHESIS REACTION‚ DECOMPOSITION AND SINGLE REAPLACEMENT REACTION) Purpose : 1. To identify the chemical changes 2. To observe the effect of temperature of a chemical reaction. Theoritical basic : A chemical reaction is a process that leads to the transformation of one set of chemical substances to another. Classically‚ chemical reactions encompass changes that strictly involve the motion of electrons in the forming and breaking of chemical bonds between
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Time Series Models for Forecasting New One-Family Houses Sold in the United States Introduction The economic recession felt in the United States since the collapse of the housing market in 2007 can be seen by various trends in the housing market. This collapse claimed some of the largest financial institutions in the U.S. such as Bear Sterns and Lehman Brothers‚ as they held over-leveraged positions in the mortgage backed securities market. Credit became widely available to unqualified borrowers
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TIME SERIES MODELS FOR FORECASTING NEW ONE-FAMILY HOUSES SOLD IN THE U.S. INTRODUCTION The housing market has been weak since its recent peak in 2005. Then‚ the sharp drop in the housing prices in 2007 contributed to the subprime loan crisis [1]. This dramatic change in the housing market not only affects the construction industry‚ but also may have a significant impact on the whole economy [3]. We are still in the midst of the housing problem with the increase in the delinquency rate and
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synthesis reaction below‚ the metal sodium reacts with chlorine gas to form sodium chloride‚ or table salt. 2Na + Cl2 → 2NaCl Because a synthesis reaction joins substances‚ the product of the reaction is more complex than the reactants. * Decomposition Reaction A
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Objective 1. To demonstrate the relationship between the pressure and temperature of saturated steam in equilibrium with water. 2. To determine the vapour pressure curve. Introduction Properties of a fluid can change when heat and work are applied to it. There’s an interchange between the fluid and its surroundings when heat and work are applied according to the study of thermodynamics. Substances such as gases and vapors are often referred as P-V-T substances. Theory: An ideal gas
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* For Refrigeration Effect RE * Enthalpy Properties h1 = 348 h2 = 400 h3 = 260 h4 = 260 * For mass in the system Qs = m (h1 – h4) 65 kW = m (348 – 260) m=0.739 kg/s EVAPORATOR * Qe = 65 kw * Temperature at the evaporator is -31.56 0C * Specific volume is 0.528 m3/kg (from ph diagram) * Consider steel material for coil: Uo=90-120 Btuhrft2 0F ; use 105Btuhrft2 0F * Allowable velocity at the liquid line is 125 – 450 ft/min; use 287.5 ft/min
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preparing a raw material containing more than 80% of fish heads‚ boiling the raw material to obtain a stock‚ removing solids and oil contents from the stock to obtain stock liquid‚ thereafter treating the stock liquid in a decomposition tank with decomposing enzyme for enzyme decomposition‚ filtering the enzyme-decomposed stock liquid to produce a clarified liquid organic fertilizer‚ and condensing the clarified liquid organic fertilizer to produce a condensed organic fertilizer. 2. The method as claimed
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slightly more viscous than water. In dilute solution‚ it appears colorless. Reactions Decomposition Hydrogen peroxide decomposes exothermically into water and oxygen gas spontaneously: 2 H2O2 → 2 H2O + O2 This process is thermodynamically favorable. It has a ΔHo of −98.2 kJ·mol−1 and a ΔS of 70.5 J·mol−1·K−1. The rate of decomposition is dependent on the temperature (cool environment slows down decomposition‚ therefore hydrogen peroxide is often stored in refrigerator) and concentration of the
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