Human Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed
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different concentrations of glucose affect osmosis in potato cells. Osmosis is the passive process of diffusing water which means within this experiment particles of water will move from a lower concentrated area to an area of higher concentration ; in this case it will be a concentration of glucose. Diffusion is when particles move from an area of high concentration to low concentration ; for example when a smell of cooking spreads through the house. Osmosis is a vital mechanism in the transport of
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with continue to occur until an equilibrium is reached. Osmosis is a different and unique kind of diffusion. Osmosis is the diffusion of water through a permeable membrane. The phrase “permeable membrane” means that the membrane will only allow specific molecules through such a water or oxygen. In Osmosis water will travel from an area of higher water potential or an area of lower water potential. Hypothesis: I think that in this lab‚ osmosis and diffusion will occur between the solutions of different
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Diffusion continues until it reaches equilibrium. Osmosis is similar to Diffusion but it’s the process in which water moves across a semi-permeable membrane and goes to the higher concentration of solute.1 2.2. Important factors to Osmosis and Diffusion include Temperature‚ Concentration and Surface area to volume ratio. Temperature can affect the rate in which a solute dissolves in a solution‚ higher temperature faster rate of Osmosis. This is caused by the molecules movements being faster
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different sucrose concentrations (0.15‚ 0.20‚ 0.25‚ 0.30‚ 0.35 and 0.50). It was expected that the potatoes would lose weight as the potatoes would have lower water potential when placed in a solution ‚ and in this can be taken from the theory of osmosis which states that “the passive diffusion of water molecules across a selectively permeable membrane down a concentration gradient” (Taylor and Bruenn‚ 2009)‚ moving water from a high to a low concentration‚ so in this case water would move from the
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How does the relationship between salt concentration effect osmosis in the aquatic plant Elodea? Quantitative data From observing the cells under a microscope‚ we see that they are continuously moving and therefore are alive. We have observed that when the cells are in the 10% sodium chloride‚ the chloroplasts are positioned to the sides of the cell wall and the cytoplasm is more expanded. Cells in the distilled water are slightly smaller than the cells in the sodium chloride solution and
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Jeffrey Shenfeld An Experimental and Statistical Analysis of SDH activity as compared in Liver‚ Kidney‚ and Heart Homogenates of the Bos taurus Methods The three tissues being analyzed in this experiment‚ those of the kidney‚ heart and liver‚ were taken from the animal Bos taurus. The tissue homogenates used were made by adding 1 gram of tissue to 20 ml of sucrose phosphate buffer. The buffer was composed of 250 mM sucrose‚ 50 mM NaPO4‚ with a pH of 7.4. This mixture was homogenized with
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The effects of osmosis on potatoes placed in different salt concentration Introduction: Osmosis is a movement of water across a semi-permeable membrane. This may only happen when there is a difference in concentration between the two sides of the membrane. The water moves from low concentrations to high concentrations. That is because the low concentrated area tries to dilute the opposite via osmosis. The effects osmosis has on a cell are either that it becomes turgid and hard as water enters
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Introduction The movement of molecules from an area of greater concentration to an area of less concentration is called Diffusion. Osmosis is the diffusion of water through a selectively permeable membrane from an area with greater concentration of water to an area with less concentration. A Hypertonic Solution is a solution of a higher concentration of solutes and a smaller concentration of water molecules than another solution. However‚ an Isotonic Solution is a solution that contains an equal
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the water and sucrose‚ osmosis can no longer take place. Osmosis can only occur when water is traveling from a higher concentrated area to a lower concentrated area. Hydrostatic pressure occurs when the bag reaches maximum capacity. Water will enter the bag through osmosis but get pushed out through hydrostatic pressure. This works in a cycle and prevents the bag from obtaining more volume and mass. 1b. The curves for the 40% and 20% sucrose differ in the rate of osmosis because of their concentration
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